Forskolin-induced clearance of the fluorescent dye sulforhodamine from rat parotid intralobular duct lumen: Visualization of the secretory function under a confocal laser scanning microscope

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	Forskolin-induced clearance of the fluorescent dye sulforhodamine from rat parotid intralobular duct lumen: Visualization of the secretory function under a confocal laser scanning microscope
	Nakamoto, T.
刊名	Journal of Membrane Biology
	2002
卷号	190 期号:3 页码:189-196
ISSN号	0022-2631
中文摘要	Cyclic AMP evokes fluid secretion with bicarbonate in exocrine ducts. Clearance of fluorescent dyes from rat parotid intralobular ducts by forskolin was visualized as a fluorescence change in the duct luminal space by optical sectioning under a confocal laser scanning microscope to clarify the secretory function in the ducts. When the isolated rat parotid intralobular duct segments were superfused with membrane-impermeable fluorescent dyes during the experimental period, fluorescent dyes were passively moved into the duct space. Forskolin and isobutylmethylxanthine decreased the fluorescence of anionic dye, sulforhodamine B, and neutral dye, dextran tetramethyl-rhodamine, in the duct space, suggesting that the forskolin-induced clearance of fluorescent dyes might be the result of fluid secretion in the ducts. Methazolamide inhibited a forskolin-induced sustained decrease in duct fluorescence and intracellular acidification. Low concentrations of external Cl-, DIDS, bumetanide and amiloride did not markedly inhibit a forskolin-induced decrease in duct fluorescence. These findings suggest that a major portion of the steady decrease in duct fluorescence by forskolin was related to intracellular HCO3- production, not the uptake mechanism of external Cl-. Glibenclamide, NPPB, DPC and DMA inhibited the forskolin-induced decrease. Forskolin evokes the clearance of fluorescent dyes from duct space possibly due to fluid secretion in rat parotid ducts, associated with secretion through CFTR and DPC-sensitive anion channels of carbonic anhydrase-dependent bi-carbonate linked with the Na+/H+ exchange mechanism.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8845]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Nakamoto, T.. Forskolin-induced clearance of the fluorescent dye sulforhodamine from rat parotid intralobular duct lumen: Visualization of the secretory function under a confocal laser scanning microscope[J]. Journal of Membrane Biology,2002,190(3):189-196.
APA	Nakamoto, T..(2002).Forskolin-induced clearance of the fluorescent dye sulforhodamine from rat parotid intralobular duct lumen: Visualization of the secretory function under a confocal laser scanning microscope.Journal of Membrane Biology,190(3),189-196.
MLA	Nakamoto, T.."Forskolin-induced clearance of the fluorescent dye sulforhodamine from rat parotid intralobular duct lumen: Visualization of the secretory function under a confocal laser scanning microscope".Journal of Membrane Biology 190.3(2002):189-196.