Three-dimensional analysis of the substrate-dependent invasive behavior of a human lung tumor cell line with a confocal laser scanning microscope

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	Three-dimensional analysis of the substrate-dependent invasive behavior of a human lung tumor cell line with a confocal laser scanning microscope
	Strohmaier, A. R.
刊名	Histochemistry and Cell Biology
	1996
卷号	105 期号:3 页码:179-185
ISSN号	0301-5564
中文摘要	Matrigel and collagen G gels were used as models for basement membrane and interstitial space-collagen, respectively, to study the invasive behavior of cells of the human lung tumor cell line EPLC 32M1, which was derived from a squamous cell carcinoma. For three dimensional analysis of the invasive process, cells were seeded onto the gels in a slide chamber and observed with a confocal laser scanning microscope. Optical sectioning in the xy and xz directions and image reconstruction with computer programs allowed us readily to obtain a three-dimensional overview of the invasive process in situ. Both types of gel showed a smooth surface. Matrigel had a granular structure whereas collagen G revealed a fiber-like morphology. The tumor cells showed a matrix-dependent behavior. On Matrigel, within 24 h of incubation, a network of cells appeared on the surface, which developed further within 72 h to interconnected multicellular cords also invading the gel. Tumor cells seeded on collagen G remained individual. They formed pseudopodia and achieved tight contact with the matrix, eventually also invading the gels in a time-dependent manner, Therefore, the composition of the substrate crucially influences the invasion path.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8842]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Strohmaier, A. R.. Three-dimensional analysis of the substrate-dependent invasive behavior of a human lung tumor cell line with a confocal laser scanning microscope[J]. Histochemistry and Cell Biology,1996,105(3):179-185.
APA	Strohmaier, A. R..(1996).Three-dimensional analysis of the substrate-dependent invasive behavior of a human lung tumor cell line with a confocal laser scanning microscope.Histochemistry and Cell Biology,105(3),179-185.
MLA	Strohmaier, A. R.."Three-dimensional analysis of the substrate-dependent invasive behavior of a human lung tumor cell line with a confocal laser scanning microscope".Histochemistry and Cell Biology 105.3(1996):179-185.