Characterization of excitability and voltage-gated ion channels of neural progenitor cells in rat hippocampus

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Characterization of excitability and voltage-gated ion channels of neural progenitor cells in rat hippocampus
	Li, T. S.
刊名	Journal of Molecular Neuroscience
	2008
卷号	35 期号:3 页码:289-295
ISSN号	0895-8696
中文摘要	We have observed the excitability of rat neural progenitor cells (NPCs) and characterized the profile of the voltage-gated ion channels, with the help of the electrophysiological measurements in NPCs in vitro, in order to clarify the electrophysiological property of NPCs. The membrane potential changes of neural progenitors were detected with fluorescent dye bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) by a confocal laser scanning microscope. The changes of fluorescent intensity of DiBAC4(3) stain after KCl stimulation were slight which indicated that the NPCs were inexcitable. Under the current-clamp, spontaneous discharge was not detected and the action potential was failed to be elicited. These findings were consistent with the result from DiBAC4(3) staining. Under the voltage clamp, the NPCs expressed two types of outward K(+) currents with no evidence for Na(+) currents. An outward delayed rectifier type K(+) current and outward transient K(+) current were elicited. Our findings demonstrate NPCs' electrophysiological properties: the electrical inexcitability indicated by the presence of two types of K(+) currents and the absence of Na(+) current.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8832]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Li, T. S.. Characterization of excitability and voltage-gated ion channels of neural progenitor cells in rat hippocampus[J]. Journal of Molecular Neuroscience,2008,35(3):289-295.
APA	Li, T. S..(2008).Characterization of excitability and voltage-gated ion channels of neural progenitor cells in rat hippocampus.Journal of Molecular Neuroscience,35(3),289-295.
MLA	Li, T. S.."Characterization of excitability and voltage-gated ion channels of neural progenitor cells in rat hippocampus".Journal of Molecular Neuroscience 35.3(2008):289-295.