Effects of the myosin ATPase inhibitor 2,3-butanedione monoxime on amyloplast kinetics and gravitropism of Arabidopsis hypocotyls

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	Effects of the myosin ATPase inhibitor 2,3-butanedione monoxime on amyloplast kinetics and gravitropism of Arabidopsis hypocotyls
	Palmieri, M.
刊名	Physiologia Plantarum
	2007
卷号	130 期号:4 页码:613-626
ISSN号	0031-9317
中文摘要	The actin cytoskeleton is a crucial component in plant gravitropism, and studies confirm that alterations to actin filaments (F-actin) can have dramatic effects on gravitropic curvature in roots and shoots. Many models for gravisensing in higher plants suggest that the key to gravity perception and signal transduction lies in intimate interactions between F-actin and amyloplasts. In this study, we investigated gravitropism in hypocotyls by analyzing the effect of myosin inhibition on gravitropic curvature in order to clarify the role of the actomyosin system in shoot gravitropism. To study amyloplast movement in endodermal cells (i.e., gravity-perceiving statocytes) of living seedlings, we repositioned a confocal laser scanning microscope (CLSM) so that its rotatable stage was oriented vertically. Seedlings containing green fluorescent protein-labeled endodermal amyloplasts were incubated with the ATPase inhibitor 2,3-butanedione monoxime (BDM) and then mounted on the stage so that the hypocotyls were vertical. Using CLSM, we imaged the endodermal amyloplasts, while the hypocotyls were oriented vertically and also after they were reoriented by 90 degrees. Our results show that BDM reduces gravitropic curvature in a concentration-dependent manner. In addition, BDM increases amyloplast movement in hypocotyls of vertical seedlings, but reduces amyloplast movement in hypocotyls of reoriented seedlings, suggesting that myosin may participate in the intracellular transport of amyloplasts in statocytes. These results can be explained in the context of amyloplasts as both noise indicators and gravity susceptors, with BDM producing less coherent amyloplast movement that results in an increased signal-to-noise ratio, which may account for at least part of the observed reduction in gravitopic curvature.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8825]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Palmieri, M.. Effects of the myosin ATPase inhibitor 2,3-butanedione monoxime on amyloplast kinetics and gravitropism of Arabidopsis hypocotyls[J]. Physiologia Plantarum,2007,130(4):613-626.
APA	Palmieri, M..(2007).Effects of the myosin ATPase inhibitor 2,3-butanedione monoxime on amyloplast kinetics and gravitropism of Arabidopsis hypocotyls.Physiologia Plantarum,130(4),613-626.
MLA	Palmieri, M.."Effects of the myosin ATPase inhibitor 2,3-butanedione monoxime on amyloplast kinetics and gravitropism of Arabidopsis hypocotyls".Physiologia Plantarum 130.4(2007):613-626.