Confocal laser scanning microscope study of terminal villi vessels in normal term and pre-eclamptic placentas

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Confocal laser scanning microscope study of terminal villi vessels in normal term and pre-eclamptic placentas
	Resta, L.
刊名	Placenta
	2006
卷号	27 期号:42162 页码:735-739
ISSN号	0143-4004
中文摘要	The aim of this study was to compare immunocytochemical confocal scanning laser microscopy measures of villus capillarization in control placentas with pre-eclamptic ones. Accordingly, placentas from normal term pregnancies (n = 3) and cases of late-onset pre-eclampsia without intrauterine growth retardation (IUGR) featuring normal uterine artery Doppler (n = 3) were analyzed by confocal scanning laser microscopy (CSLM), which is a powerful technique for obtaining three-dimensional reconstructions of any kind of blood vessels (arteries, veins, capillaries). A laser light beam is used in order to detect CD34 antibody-related immunofluorescence, which is a marker of endothelial cells. Villus capillarization was assessed by estimating the following parameters: number of pixels, mean, maximum and minimum immunofluorescence amplitude. Our results show a significant hyper-ramification of the capillary loop in pre-eclamptic placentas, featuring irregular profile and narrow lumina. Such findings support the hypothesis that several agents causing angiogenesis and vasoconstriction affect villus vessels in pre-eclamptic placentas, thus promoting a lasting condition of fetal hypoxia by decreasing endothelial surface and materno-fetal exchanges.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8789]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Resta, L.. Confocal laser scanning microscope study of terminal villi vessels in normal term and pre-eclamptic placentas[J]. Placenta,2006,27(42162):735-739.
APA	Resta, L..(2006).Confocal laser scanning microscope study of terminal villi vessels in normal term and pre-eclamptic placentas.Placenta,27(42162),735-739.
MLA	Resta, L.."Confocal laser scanning microscope study of terminal villi vessels in normal term and pre-eclamptic placentas".Placenta 27.42162(2006):735-739.