DISTRIBUTION OF MICROTUBULES DURING THE GROWTH OF TOBACCO POLLEN TUBES | |
Delcasino, C. | |
刊名 | Biology of the Cell |
1993 | |
卷号 | 79期号:2页码:125-132 |
ISSN号 | 0248-4900 |
中文摘要 | The distribution of microtubules was investigated in Nicotiana tabacum pollen tubes at different stages of tube growth by immunofluorescence microscopy. Using specific antibodies, the presence of microtubules consisting of different tubulin isoforms was tested. alpha-, beta- and tyrosinated alpha-tubulin were present within the tube, whereas the acetylated form was lacking. The presence of tubulin subunits in pollen tube extracts was also investigated by immunoblotting analyses. The use of a confocal laser scanning microscope integrated with computer-assisted imaging, allowed a detailed visualization of the microtubule distribution and organization. Cytoplasmic microtubules organized as short bundles with various orientations were detected at the apex of long tubes. |
语种 | 英语 |
公开日期 | 2015-06-08 |
内容类型 | 期刊论文 |
源URL | [http://ir.xtbg.org.cn/handle/353005/8764] |
专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
推荐引用方式 GB/T 7714 | Delcasino, C.. DISTRIBUTION OF MICROTUBULES DURING THE GROWTH OF TOBACCO POLLEN TUBES[J]. Biology of the Cell,1993,79(2):125-132. |
APA | Delcasino, C..(1993).DISTRIBUTION OF MICROTUBULES DURING THE GROWTH OF TOBACCO POLLEN TUBES.Biology of the Cell,79(2),125-132. |
MLA | Delcasino, C.."DISTRIBUTION OF MICROTUBULES DURING THE GROWTH OF TOBACCO POLLEN TUBES".Biology of the Cell 79.2(1993):125-132. |
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