Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis

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	Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis
	Kim, S.
刊名	Water Research
	2013
卷号	47 期号:13 页码:4403-4411
ISSN号	0043-1354
中文摘要	The effects of UV-assisted TiO2-photocatalytic oxidation (PCO) inactivation of pathogenic bacteria (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium) in a liquid culture using different domains of UV irradiation (A, B and C) were evaluated. Structural changes in super-coiled plasmid DNA (pUC19) and genomic DNA of E. coli were observed using gel electrophoresis to demonstrate the photodynamic DNA strand breaking activity of UV-assisted TiO2-PCO. Membrane damage in bacterial cells was observed using both a scanning electron microscope (SEM) and a confocal laser scanning microscope (CLSM). Both UVC-TiO2-PCO and UVC alone resulted in an earlier bactericidal phase (initial counts of approximately 6 log CFU/mL) in 60 s and 90 s, respectively, in liquid culture. UVC-TiO2-PCO treatment for 6 min converted all plasmid DNA to the linear form; however, under UVC irradiation alone, super-coiled DNA remained. Prolonged UVC-TiO2-PCO treatment resulted in structural changes in genomic DNA from E. coli. SEM observations revealed that bacteria suffered severe visible cell damage after UVC-TiO2-PCO treatment for 30-60 mm. S. typhimurium cells showed visible damage after 30 min, which was confirmed using CLSM. All treated cells were stained red using propidium iodide under a fluorescent light. (C) 2013 Elsevier Ltd. All rights reserved.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8760]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Kim, S.. Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis[J]. Water Research,2013,47(13):4403-4411.
APA	Kim, S..(2013).Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis.Water Research,47(13),4403-4411.
MLA	Kim, S.."Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis".Water Research 47.13(2013):4403-4411.