Specific localization of gap junction protein, connexin45, in the deep muscular plexus of dog and rat small intestine

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Specific localization of gap junction protein, connexin45, in the deep muscular plexus of dog and rat small intestine
	Nakamura, K.
刊名	Cell and Tissue Research
	1998
卷号	292 期号:3 页码:487-494
ISSN号	0302-766X
中文摘要	Cellular networks of pacemaker activity in intestinal movements are still a matter of debate. Because gap-junctional intercellular communication in the intestinal wall may provide important clues for understanding regulatory mechanisms of intestinal movements, we have attempted to clarify the distribution patterns of three types of gap junction proteins. Using antibodies for connexin40, connexin43, connexin45, smooth muscle actin, and vimentin, immunocytochemical observations were made with the confocal laser scanning microscope on cryosections of fresh-frozen small intestine and colon of the dog and rat. Connexin 45 was localized along the deep muscular plexus of the small intestine in both dog and rat. Double labeling studies revealed that connexin45 overlapped with vimentin-, but not actin-positive areas, indicating the fibroblast-like nature of the cells, rather than their being smooth muscle-like. Connexin43 immunoreactivity appeared along the smooth muscle cell surface in the outer circular layer of the small intestine of both animals. Connexin 40 immunoreactivity was not observed in the muscle layer other than in the wall of large blood vessels. It is suggested that connexin45-expressing cells along the deep muscular plexus of dog and rat small intestine are likely to act as a constituent of a pacemaker system, which may include a conductive system, by forming a cellular network operating via specific types of gap junctions.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8758]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Nakamura, K.. Specific localization of gap junction protein, connexin45, in the deep muscular plexus of dog and rat small intestine[J]. Cell and Tissue Research,1998,292(3):487-494.
APA	Nakamura, K..(1998).Specific localization of gap junction protein, connexin45, in the deep muscular plexus of dog and rat small intestine.Cell and Tissue Research,292(3),487-494.
MLA	Nakamura, K.."Specific localization of gap junction protein, connexin45, in the deep muscular plexus of dog and rat small intestine".Cell and Tissue Research 292.3(1998):487-494.