Urocortin II inhibits the apoptosis of mesenteric arterial smooth muscle cells via L-type calcium channels in spontaneously hypertensive rats

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	Urocortin II inhibits the apoptosis of mesenteric arterial smooth muscle cells via L-type calcium channels in spontaneously hypertensive rats
	Tao, J.
刊名	Cellular Physiology and Biochemistry
	2006
卷号	17 期号:42067 页码:111-120
ISSN号	1015-8987
中文摘要	Urocortin (UCN) II, a newly isolated corticotropin-releasing-factor (CRF) related peptide, has been found to have potent cardiovascular protective effects. To investigate the mechanisms of its vascular protective effects, we exposed mesenteric arterial smooth muscle cells (MASMC) from spontaneously hypertensive rats (SHR) to UCN II to observe the change in cell apoptosis using TUNEL assay and measured intracellular calcium concentration ([Ca2+](j)) using confocal laser scanning microscope. In addition, effects of UCN II on L-type calcium currents (I-Ca,I-L) were also measured using whole-cell patch clamp. Our results showed that UCN II concentration-dependently, but time-independently inhibited cell apoptosis. Astressin 2B, a special CRF 2 receptor antagonist, had no influence on this inhibition. Hypoxia or Bay K8644, the L-type calcium channel activator, induced the apoptosis of MASMC from SHR. Pretreatment of the cells with UCN II diminished the effects of hypoxia or Bay K8644. UCN II was also observed to reduce Ca2+](j) increase induced by KCl or Bay K8644. UCN II concentration-dependently inhibited I-Ca,I-L, which was not affected by astressin 2B. It did not affect the activation of I-Ca,I-L, but markedly shifted the inactivation curve to the left. In conclusion, UCN II inhibits the apoptosis of MASMC from SHR via inhibiting L-type calcium channels. Copyright (c) 2006 S. Karger AG, Basel.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8752]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Tao, J.. Urocortin II inhibits the apoptosis of mesenteric arterial smooth muscle cells via L-type calcium channels in spontaneously hypertensive rats[J]. Cellular Physiology and Biochemistry,2006,17(42067):111-120.
APA	Tao, J..(2006).Urocortin II inhibits the apoptosis of mesenteric arterial smooth muscle cells via L-type calcium channels in spontaneously hypertensive rats.Cellular Physiology and Biochemistry,17(42067),111-120.
MLA	Tao, J.."Urocortin II inhibits the apoptosis of mesenteric arterial smooth muscle cells via L-type calcium channels in spontaneously hypertensive rats".Cellular Physiology and Biochemistry 17.42067(2006):111-120.