Interaction of peroxisomes with microtubules - In vitro studies using a novel peroxisome-microtubule binding assay

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	Interaction of peroxisomes with microtubules - In vitro studies using a novel peroxisome-microtubule binding assay
	Thiemann, M.
刊名	European Journal of Biochemistry
	2000
卷号	267 期号:20 页码:6264-6275
ISSN号	0014-2956
中文摘要	The association of membrane-bounded cell organelles to microtubules is crucial for determination of their shape, intracellular localization and translocation. We have shown previously the high affinity binding of peroxisomes to microtubules which appears to be of static nature as in vivo studies indicate that only a few peroxisomes move along the microtubular tracks. In order to characterize the interactions of peroxisomes with microtubules, we have developed a semiquantitative in vitro binding assay, which is based on the association of highly purified rat liver peroxisomes to microtubules coated onto microtiterplates. The binding was visualized by differential interference contrast and immunofluorescence using a confocal laser scanning microscope. The binding was concentration dependent and saturable, being affected by time, temperature, and pH. Addition of ATP or the motor proteins kinesin and dynein increased the binding capacity, while ATP-depletion or microtubule associated proteins (MAPs) decreased it. KCl treatment of peroxisomes reduced the binding, which was restored by dialyzed KCl-stripping eluate as well as by rat liver cytosol. The reconstituting effect of cytosol was abolished by its pretreatment with proteases or N-ethylmaleimide. Moreover, the treatment of peroxisomes with proteases or N-ethylmaleimide reduced their binding, which was not reversed by cytosol. These results suggest the involvement of a peroxisomal membrane protein and cytosolic factor(s) in the binding of peroxisomes to microtubules. This notion is supported by the observation that distinct subfractions of dialyzed KCl-stripping eluate obtained by gel chromatography augmented the binding. Those subfractions, as well as purified peroxisome fractions, exhibited strong immunoreactivity with an antibody to cytoplasmic linker protein (CLIP)-115, revealing a 70-kDa polypeptide. Moreover, immunodepletion of KCl-stripping eluate and its subfractions with an antibody to the conserved microtubule binding domain of CLIPs, abolished their promoting effect on the binding, thus suggesting the involvement of a CLIP-related protein in the binding of peroxisomes to microtubules.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8747]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Thiemann, M.. Interaction of peroxisomes with microtubules - In vitro studies using a novel peroxisome-microtubule binding assay[J]. European Journal of Biochemistry,2000,267(20):6264-6275.
APA	Thiemann, M..(2000).Interaction of peroxisomes with microtubules - In vitro studies using a novel peroxisome-microtubule binding assay.European Journal of Biochemistry,267(20),6264-6275.
MLA	Thiemann, M.."Interaction of peroxisomes with microtubules - In vitro studies using a novel peroxisome-microtubule binding assay".European Journal of Biochemistry 267.20(2000):6264-6275.