Quantification of protein A-gold staining for peroxisomal enzymes by confocal laser scanning microscopy

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	Quantification of protein A-gold staining for peroxisomal enzymes by confocal laser scanning microscopy
	Ogiwara, N.
刊名	Journal of Histochemistry & Cytochemistry
	1999
卷号	47 期号:10 页码:1343-1349
ISSN号	0022-1554
中文摘要	The protein A-gold technique has been widely applied for visual localization and quantification of various antigens by electron microscopy. Observation of specimens stained by the protein A-gold technique with conventional light microscopy is difficult because of insufficient sensitivity of the staining. Light microscopic visualization and quantification of the reaction products were attempted employing a confocal laser scanning microscope (CLSM). Liver tissues of normal and peroxisome proliferator-treated rats were fixed and embedded in Lowicryl K4M resin. Ultrathin and thin sections were stained for catalase and a peroxisome-specific beta-oxidation enzyme by the protein A-gold technique. Ultrathin sections were observed by electron microscopy and the labeling density for each enzyme was analyzed with an image analyzer. Thin sections were observed with a CLSM in the reflection mode and the intensity of the light reflection was analyzed under the same conditions for all specimens. A comparison of these two observation procedures was also attempted using liver tissues stained with various concentrations of the antibody for catalase. The intensity of the reflection for each, as observed by CLSM, correlated well with the labeling density observed by electron microscopy. CLSM made it possible to quantify and to directly observe protein A-gold staining at the light microscopic level.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8730]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Ogiwara, N.. Quantification of protein A-gold staining for peroxisomal enzymes by confocal laser scanning microscopy[J]. Journal of Histochemistry & Cytochemistry,1999,47(10):1343-1349.
APA	Ogiwara, N..(1999).Quantification of protein A-gold staining for peroxisomal enzymes by confocal laser scanning microscopy.Journal of Histochemistry & Cytochemistry,47(10),1343-1349.
MLA	Ogiwara, N.."Quantification of protein A-gold staining for peroxisomal enzymes by confocal laser scanning microscopy".Journal of Histochemistry & Cytochemistry 47.10(1999):1343-1349.