An apical tension-sensitive microfilament system in retinal pigment epithelial cells

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	An apical tension-sensitive microfilament system in retinal pigment epithelial cells
	Nagai, H.
刊名	Experimental Cell Research
	1996
卷号	223 期号:1 页码:63-71
ISSN号	0014-4827
中文摘要	A tension-sensitive microfilament (RIF) system in the plane of zonula adherens (ZA) junctions of retinal pigment epithelial (RPE) cells is described. Organ cultures and whole mounts of RPE from chick embryos were labeled for F-actin and examined with a confocal laser scanning microscope. The changes in the distribution of MFs during alterations in the direction of tensional stress, generated in the organ culture system or induced by mechanical wounding, were examined. In RPE cells throughout the organ culture, fine MF bundles, termed apical stress fibers (ASF), oriented parallel to the direction in which tension had been generated, were observed at the level of circumferential MF bundles (CMBs) associated with ZA junctions. Within 24 h after the direction of the tension was experimentally altered by 90 degrees, the orientation of ASF also changed, becoming aligned along the new lines of the tension. The ASF likewise react to changes in the direction of stress generated when RPE cells respond to wounding in organ culture. The presence of similar MFs in RPE cells in the posterior retina of 13-day chick embryos in situ may reflect mechanical stretching of the RPE cells that occurs during the development of the eye. The ASF probably limit the stretching of the CMBs of RPE cells in any one direction, thereby protecting the RPE cells against tensional stress. This system of MFs may be especially important in chick RPE cells, which lack desmosomes and keratin-type intermediate filaments that normally have this function in Other cell types. (C) 1996 Academic Press, Inc.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8728]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Nagai, H.. An apical tension-sensitive microfilament system in retinal pigment epithelial cells[J]. Experimental Cell Research,1996,223(1):63-71.
APA	Nagai, H..(1996).An apical tension-sensitive microfilament system in retinal pigment epithelial cells.Experimental Cell Research,223(1),63-71.
MLA	Nagai, H.."An apical tension-sensitive microfilament system in retinal pigment epithelial cells".Experimental Cell Research 223.1(1996):63-71.