Gas phase activity of anti-FITC antibodies immobilized on a surface acoustic wave resonator device

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Gas phase activity of anti-FITC antibodies immobilized on a surface acoustic wave resonator device
	Stubbs, D. D.
刊名	Biosensors & Bioelectronics
	2002
卷号	17 期号:42162 页码:471-477
ISSN号	0956-5663
中文摘要	In this paper we present the results of a series of experiments on the activity of antibodies in a vapor phase sensor. For these experiments the sensor component was a ST-Quartz resonator with a center frequency of approximately 250 MHz. Anti-FITC antibodies were attached to the electrodes on the device surface via a protein-A crosslinker. Surface acoustic wave (SAW) resonator devices with various coatings were mounted in TO-8 packages, inserted into our sensor head module and subjected to various fluorescent analyte gases. Numerous controls were performed including the use of coated and uncoated devices along with devices coated with antibodies which were not specific for the target analyte. The SAW immunosensor response was monitored and a baseline frequency shift was observed when the analyte being presented was the antigen for the immobilized antibody. To provide an independent measure of antibody/antigen binding, the devices were removed from the sensor head, washed with a buffer solution to remove any unbound analyte, and then inspected using a confocal laser scanning microscope (CLSM). Since all the analytes being used in these experiments were fluorescent this afforded us the opportunity to visualize the attachment of the analyte to the antibody film. Given the high resolution of the CLSM, we were able to identify the location of the attachment of the fluorescent analytes relative to the 1.5 mum wide electrodes of the SAW device. We believe that these experiments demonstrate that we have achieved real time molecular recognition of these small molecules in the vapor phase. (C) 2002 Elsevier Science B.V. All rights reserved.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8724]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Stubbs, D. D.. Gas phase activity of anti-FITC antibodies immobilized on a surface acoustic wave resonator device[J]. Biosensors & Bioelectronics,2002,17(42162):471-477.
APA	Stubbs, D. D..(2002).Gas phase activity of anti-FITC antibodies immobilized on a surface acoustic wave resonator device.Biosensors & Bioelectronics,17(42162),471-477.
MLA	Stubbs, D. D.."Gas phase activity of anti-FITC antibodies immobilized on a surface acoustic wave resonator device".Biosensors & Bioelectronics 17.42162(2002):471-477.