Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus)

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	Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus)
	Wang, Xianlei 1,2; Tan, Xungang1 ; Zhang, Pei-Jun 1; Zhang, Yuqing 1; Xu, Peng1
刊名	JOURNAL OF BIOSCIENCES
	2014-12-01
卷号	39 期号:5 页码:849-858
关键词	Flounder RAG1 RAG2 thymus
通讯作者	Tan, XG (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	During the development of B and T lymphocytes, Ig and TCR variable region genes are assembled from germline V, D, and J gene segments by a site-specific recombination reaction known as V(D)J recombination. The process of somatic V(D)J recombination, mediated by the recombination-activating gene (RAG) products, is the most significant characteristic of adaptive immunity in jawed vertebrates. Flounder (Paralichthys olivaceus) RAG1 and RAG2 were isolated by Genome Walker and RT-PCR, and their expression patterns were analysed by RT-PCR and in situ hybridization on sections. RAG1 spans over 7.0 kb, containing 4 exons and 3 introns, and the full-length ORF is 3207 bp, encoding a peptide of 1068 amino acids. The first exon lies in the 5'-UTR, which is an alternative exon. RAG2 full-length ORF is 1062 bp, encodes a peptide of 533 amino acids, and lacks introns in the coding region. In 6-month-old flounders, the expression of RAG1 and RAG2 was essentially restricted to the pronephros (head kidney) and mesonephros (truck kidney). Additionally, both of them were mainly expressed in the thymus. These results revealed that the thymus and kidney most likely serve as the primary lymphoid tissues in the flounder.
学科主题	Life Sciences & Biomedicine - Other Topics
收录类别	SCI
语种	英语
WOS记录号	WOS:000345809200012
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24157]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋环流与波动重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xianlei,Tan, Xungang,Zhang, Pei-Jun,et al. Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus)[J]. JOURNAL OF BIOSCIENCES,2014,39(5):849-858.
APA	Wang, Xianlei,Tan, Xungang,Zhang, Pei-Jun,Zhang, Yuqing,&Xu, Peng.(2014).Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus).JOURNAL OF BIOSCIENCES,39(5),849-858.
MLA	Wang, Xianlei,et al."Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus)".JOURNAL OF BIOSCIENCES 39.5(2014):849-858.