Quantitative proteomic analysis of thylakoid from two microalgae (Haematococcus pluvialis and Dunaliella salina) reveals two different high light-responsive strategies

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	Quantitative proteomic analysis of thylakoid from two microalgae (Haematococcus pluvialis and Dunaliella salina) reveals two different high light-responsive strategies
	Gu, Wenhui 1; Li, Huan 1,2; Zhao, Peipei1,2 ; Yu, Ruixue 3; Pan, Guanghua 3; Gao, Shan 1; Xie, Xiujun 1; Huang, Aiyou 1; He, Linwen 1; Wang, Guangce 1
刊名	SCIENTIFIC REPORTS
	2014-10-22
卷号	4 页码:6661
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Under high light (HL) stress, astaxanthin-accumulating Haematococcus pluvialis and beta-carotene-accumulating Dunaliella salina showed different responsive patterns. To elucidate cellular-regulating strategies photosynthetically and metabolically, thylakoid membrane proteins in H. pluvialis and D. salina were extracted and relatively quantified after 0 h, 24 h and 48 h of HL stress. Proteomic analysis showed that three subunits of the cytochrome b(6)/f complex were greatly reduced under HL stress in H. pluvialis, while they were increased in D. salina. Additionally, the major subunits of both photosystem (PS) II and PSI reaction center proteins were first reduced and subsequently recovered in H. pluvialis, while they were gradually reduced in D. salina. D. salina also showed a greater ability to function using the xanthophyll-cycle and the cyclic photosynthetic electron transfer pathway compared to H. pluvialis. We propose a reoriented and effective HL-responsive strategy in H. pluvialis, enabling it to acclimate under HL. The promising metabolic pathway described here contains a reorganized pentose phosphate pathway, Calvin cycle and glycolysis pathway participating in carbon sink formation under HL in H. pluvialis. Additionally, the efficient carbon reorientation strategy in H. pluvialis was verified by elevated extracellular carbon assimilation and rapid conversion into astaxanthin.
学科主题	Science & Technology - Other Topics
收录类别	SCI
语种	英语
WOS记录号	WOS:000343589400002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24155]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Gu, Wenhui,Li, Huan,Zhao, Peipei,et al. Quantitative proteomic analysis of thylakoid from two microalgae (Haematococcus pluvialis and Dunaliella salina) reveals two different high light-responsive strategies[J]. SCIENTIFIC REPORTS,2014,4:6661.
APA	Gu, Wenhui.,Li, Huan.,Zhao, Peipei.,Yu, Ruixue.,Pan, Guanghua.,...&Wang, Guangce.(2014).Quantitative proteomic analysis of thylakoid from two microalgae (Haematococcus pluvialis and Dunaliella salina) reveals two different high light-responsive strategies.SCIENTIFIC REPORTS,4,6661.
MLA	Gu, Wenhui,et al."Quantitative proteomic analysis of thylakoid from two microalgae (Haematococcus pluvialis and Dunaliella salina) reveals two different high light-responsive strategies".SCIENTIFIC REPORTS 4(2014):6661.