PtSerpin from the swimming crab Portunus trituberculatus, a putative regulator of prophenoloxidase activation with antibacterial activity

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	PtSerpin from the swimming crab Portunus trituberculatus, a putative regulator of prophenoloxidase activation with antibacterial activity
	Liu, Yuan 1; Shi, Guohui1,3 ; Cui, Zhaoxia 1,2; Luo, Danli 1,3; Song, Chengwen1,3 ; Li, Xihong 1,3; Hui, Min 1; Li, Yingdong 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014-08-01
卷号	39 期号:2 页码:365-371
关键词	Portunus trituberculatus Serpin Bacterial growth inhibition Prophenoloxidase activating system
通讯作者	Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Serpin or serine protease inhibitor is the largest family of protease inhibitors involved in many innate immune pathways, particularly the prophenoloxidase (proPO) activating system in arthropod. Here, we report the molecular and functional characterization of PtSerpin identified from the swimming crab Portunus trituberculatus. The genomic sequence encoding mature peptide of PtSerpin gene contained two exons of 84 and 1098 bp separated by one intron of 111 bp. The recombinant PtSerpin (rPtSerpin) with a predicted size of 44 kDa was expressed in Escherichia coli system, purified and assayed for its activities. The rPtSerpin exhibited inhibitory activity against trypsin in a dose-dependent manner, but did not affect chymotrypsin, which could define a role for PtSerpin as a trypsin inhibitor. The rPtSerpin could inhibit the growth of Gram-negative bacterium Vibrio alginolyticus, but not the tested Gram-positive bacterium and fungus. Further phenoloxidase (PO) assay showed PO activity was dramatically increased in hemocyte lysate supernatant of P. trituberculatus upon bacterial challenge. The rPtSerpin could depress the crab proPO system activation in vitro, and it could lead to 100% inhibition of PO activity under the concentration of 8.62 mu M. Moreover, the rPtSerpin was able to inhibit the PO activity induced by rPtcSP and rPtSPH1. These results together indicate that PtSerpin is a potential trypsin inhibitor and may participate in crab innate immunity by the inhibition of bacterial growth and the regulation of proPO system. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000339773000031
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24153]
专题	海洋研究所_海洋生态与环境科学重点实验室海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuan,Shi, Guohui,Cui, Zhaoxia,et al. PtSerpin from the swimming crab Portunus trituberculatus, a putative regulator of prophenoloxidase activation with antibacterial activity[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):365-371.
APA	Liu, Yuan.,Shi, Guohui.,Cui, Zhaoxia.,Luo, Danli.,Song, Chengwen.,...&Li, Yingdong.(2014).PtSerpin from the swimming crab Portunus trituberculatus, a putative regulator of prophenoloxidase activation with antibacterial activity.FISH & SHELLFISH IMMUNOLOGY,39(2),365-371.
MLA	Liu, Yuan,et al."PtSerpin from the swimming crab Portunus trituberculatus, a putative regulator of prophenoloxidase activation with antibacterial activity".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):365-371.