Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens

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	Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens
	Zhang, Shu-ren 1,2; Zhang, Lu 1,2; Sun, Li 1
刊名	DISEASES OF AQUATIC ORGANISMS
	2014-08-11
卷号	110 期号:3 页码:181-191
关键词	Pseudomonas fluorescens TonB-dependent receptor Iron Virulence Vaccine
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Pseudomonas fluorescens is a Gram-negative bacterium that can infect a wide range of farmed fish. However, very little is known about the virulence mechanism of P. fluorescens as a fish pathogen. In this study, we identified and analyzed 3 TonB-dependent outer membrane receptors (TDRs) from a pathogenic P. fluorescens strain isolated from fish. In silico analysis revealed that all 3 proteins (named Tdr1 to 3) possess structural domains typical of TDRs. Quantitative real time RT-PCR analysis showed that tdr1, tdr2, and tdr3 expressions were upregulated under iron-depleted conditions. Compared to the wild type, mutants defective in tdr1, tdr2, and tdr3 were retarded in growth to different extents. Infection in a turbot Scophthalmus maximus model showed that all 3 mutants were impaired in their ability to desseminate into and colonize host tissues. In addition, the tdr1 and tdr3 mutants exhibited significantly reduced virulence. When used as subunit vaccines, purified recombinant proteins of Tdr1, Tdr2, and, in particular, Tdr3 elicited significant protection in turbot against lethal P. fluorescens challenge. The vaccinated fish produced specific serum antibodies, which, when incubated with P. fluorescens, blocked infection of P. fluorescens in fish cells. Together these results indicate that Tdr1, Tdr2, and Tdr3 are iron-regulated factors that participate in bacterial virulence and induce protective immunity as subunit vaccines.
学科主题	Fisheries; Veterinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000342923400003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24122]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Shu-ren,Zhang, Lu,Sun, Li. Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens[J]. DISEASES OF AQUATIC ORGANISMS,2014,110(3):181-191.
APA	Zhang, Shu-ren,Zhang, Lu,&Sun, Li.(2014).Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens.DISEASES OF AQUATIC ORGANISMS,110(3),181-191.
MLA	Zhang, Shu-ren,et al."Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens".DISEASES OF AQUATIC ORGANISMS 110.3(2014):181-191.