Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production

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	Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production
	Gao, Yongqiang 1,2; Liu, Changshui 1,2; Ding, Yamei 3; Sun, Chao 1; Zhang, Rubing 1; Xian, Mo 1,4; Zhao, Guang 1,4
刊名	PLOS ONE
	2014-05-16
卷号	9 期号:5 页码:e97845
通讯作者	Xian, M (reprint author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China.
英文摘要	Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In our previous study, a pathway for P3HP production was constructed in recombinant Esecherichia coli. Seven exogenous genes in P3HP synthesis pathway were carried by two plasmid vectors. However, the P3HP production was severely suppressed by strain instability due to plasmid loss. In this paper, two strategies, chromosomal gene integration and plasmid addiction system (PAS) based on amino acid anabolism, were applied to construct a genetically stable strain. Finally, a combination of those two methods resulted in the best results. The resultant strain carried a portion of P3HP synthesis genes on chromosome and the others on plasmid, and also brought a tyrosine-auxotrophy based PAS. In aerobic fed-batch fermentation, this strain produced 25.7 g/L P3HP from glycerol, about 2.5-time higher than the previous strain with two plasmids. To the best of our knowledge, this is the highest P3HP production from inexpensive carbon sources.
学科主题	Science & Technology - Other Topics
收录类别	SCI
语种	英语
WOS记录号	WOS:000339614800076
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24102]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Collaborat Innovat Ctr Marine Biomass Fibers Mat, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yongqiang,Liu, Changshui,Ding, Yamei,et al. Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production[J]. PLOS ONE,2014,9(5):e97845.
APA	Gao, Yongqiang.,Liu, Changshui.,Ding, Yamei.,Sun, Chao.,Zhang, Rubing.,...&Zhao, Guang.(2014).Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production.PLOS ONE,9(5),e97845.
MLA	Gao, Yongqiang,et al."Development of Genetically Stable Escherichia coli Strains for Poly(3-Hydroxypropionate) Production".PLOS ONE 9.5(2014):e97845.