Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus

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	Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus
	Jiang, Hao 1,2; Li, Fuhua 1; Zhang, Jiquan ; Zhang, Jinkang 2; Huang, Bingxin 1; Yu, Yang1,2 ; Xiang, Jianhai1
刊名	MARINE BIOTECHNOLOGY
	2014-02-01
卷号	16 期号:1 页码:111-123
关键词	Vibrio WSSV Shrimp Proteomics 2-DE
通讯作者	Xiang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
英文摘要	Fenneropenaeus chinensis (Chinese shrimp) culture industry, like other Penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. To better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. Firstly, the soluble hepatopancreas protein samples in adult Chinese shrimp among control, heat-inactivated Vibrio-challenged and white spot syndrome virus-infected groups were separated by 2-DE (pH range, 4-7; sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and pH range, 3-10; tricine-SDS-PAGE). Then the differentially expressed protein spots (a parts per thousand yen1.5-fold or a parts per thousand currency sign0.67-fold averagely of controls) were analyzed by LC-ESI-MS/MS. Using Mascot online database searching algorithm and SEQUEST searching program, 48 and 49 differentially expressed protein spots were successfully identified in response to Vibrio and white spot syndrome virus infection, respectively. Based on these results, we discussed the mechanism of immune response of the shrimp and shed light on the differences between immune response of shrimp toward Vibrio and white spot syndrome virus. This study also set a basis for further analyses of some key genes in immune response of Chinese shrimp.
学科主题	Biotechnology & Applied Microbiology; Marine & Freshwater Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000330433700011
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24094]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Hao,Li, Fuhua,Zhang, Jiquan,et al. Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus[J]. MARINE BIOTECHNOLOGY,2014,16(1):111-123.
APA	Jiang, Hao.,Li, Fuhua.,Zhang, Jiquan.,Zhang, Jinkang.,Huang, Bingxin.,...&Xiang, Jianhai.(2014).Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus.MARINE BIOTECHNOLOGY,16(1),111-123.
MLA	Jiang, Hao,et al."Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus".MARINE BIOTECHNOLOGY 16.1(2014):111-123.