Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker
	Cui, Yulin 1,2; Qin, Song 1; Jiang, Peng 2
刊名	PLOS ONE
	2014-06-09
卷号	9 期号:6 页码:e98607
通讯作者	Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Shandong, Peoples R China.
英文摘要	The objective of this research was to establish a chloroplast transformation technique for Platymonas (Tetraselmis) subcordiformis. Employing the gfp gene as a reporter and the bar gene as a selectable marker, transformation vectors of P. subcordiformis chloroplast were constructed with endogenous fragments rrn16S-trnl (left) and trnA-rrn23S (right) as a recombination site of the chloroplast genome. The plasmids were transferred into P. subcordiformis via particle bombardment. Confocal laser scanning microscopy indicated that the green fluorescence protein was localized in the chloroplast of P. subcordiformis, confirming the activity of the Chlamydomonas reinhardtii promoter. Cells transformed with the bar gene were selected using the herbicide Basta. Resistant colonies were analyzed by PCR and Southern blotting, and the results indicated that the bar gene was successfully integrated into the chloroplast genome via homologous recombination. The technique will improve genetic engineering of this alga.
学科主题	Science & Technology - Other Topics
收录类别	SCI
语种	英语
WOS记录号	WOS:000337165600008
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24087]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Cui, Yulin,Qin, Song,Jiang, Peng. Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker[J]. PLOS ONE,2014,9(6):e98607.
APA	Cui, Yulin,Qin, Song,&Jiang, Peng.(2014).Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker.PLOS ONE,9(6),e98607.
MLA	Cui, Yulin,et al."Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker".PLOS ONE 9.6(2014):e98607.