Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri

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	Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri
	Shi, Xiaowei 1,2; Wang, Lingling 1; Zhou, Zhi 1; Liu, Rui 1; Li, Yunchen 2; Song, Linsheng 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014-05-01
卷号	38 期号:1 页码:204-210
关键词	Chlamys farreri Acetylcholine (ACh) Lipopolysaccharide (LPS) Tumor necrosis factor alpha (TNF-alpha) Immunomodulation
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
英文摘要	Acetylcholine (ACh) is an indispensable neurotransmitter and neuromodulator in the cholinergic nervous system and it is implicated in the dynamic modulation of immune response in vertebrates. Although ACh has also been identified in most invertebrates, the knowledge about its immunomodulation is still limited. In the present study, the immunoreactivities of ACh and acetylcholinesterase (AChE) were observed in all the tested tissues of scallop Chlamys farreri, including adductor muscle, mantle, gill, hepatopancreas, kidney and gonad. The ACh concentration in the supernate of scallop hemolymph increased from 11.59 +/- 0.27 to 14.36 +/- 0.17 mu L-1 at 6 h after LPS (0.5 mg ml(-1)) stimulation, and increased to 15.51 +/- 1.20 mu M L-1 at 3 h after the stimulation of tumor necrosis factor alpha (TNF-alpha) (50 ng ml(-1)). After LPS stimulation, the mRNA expression levels of superoxide dismutase (CfSOD), catalase (CfCAT) and lysozyme (CfLYZ) in hemocytes increased significantly at 3 h (P < 0.05), 6 h (P < 0.05) and 12 h (P < 0.05), respectively. Compared with the LPS treatment, the induction of CfSOD, CfCAT and CfLYZ expression in hemocytes was repressed effectively (P < 0.05) by the co-stimulation of LPS and ACh (5 x 10(-7) M) at 3 h (P < 0.05), 6 h < 0.05) and 12 h (P < 0.05), respectively. Furthermore, the expression level of CfCAT in hemocytes increased significantly after 12 h by the co-stimulation with LPS and ACh (P < 0.05). These results indicated collectively that the scallop cholinergic nervous system could be activated by immune stimulations, and it might play an essential role in immunomodulation of scallops. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000336353700027
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24065]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Linyi Univ, Coll Phys Educ, Linyi 276000, Peoples R China
推荐引用方式 GB/T 7714	Shi, Xiaowei,Wang, Lingling,Zhou, Zhi,et al. Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):204-210.
APA	Shi, Xiaowei,Wang, Lingling,Zhou, Zhi,Liu, Rui,Li, Yunchen,&Song, Linsheng.(2014).Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,38(1),204-210.
MLA	Shi, Xiaowei,et al."Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):204-210.