Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila

	Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila
	Liu, Mei 1; Wang, Baojie 1; Jiang, Keyong 1; Gong, Kui 1; Sun, Shujuan 1; Wang, Lei 1; Liu, Wenzhen 2; Fu, Yaping 2
刊名	JOURNAL OF THE WORLD AQUACULTURE SOCIETY
	2014-06-01
卷号	45 期号:3 页码:269-278
通讯作者	Wang, L (reprint author), Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Penaeidin is an antimicrobial peptide family found in penaeid shrimp. Penaeidin3 has a broad spectrum of antimicrobial activity and is most effective against certain bacterial species. In this study, the penaeidin3-2 gene was cloned from the Chinese shrimp, Fenneropenaeus chinensis. It was then transformed into rice plants using an Agrobacterium-mediated transformation system. The transformants were verified by polymerase chain reaction (PCR) sequencing and real-time polymerase chain reaction (RT-PCR) analysis. To evaluate the application of penaeidin3-2 expressed by rice plants in the feedstock industry and aquaculture practice, the transgenic rice bran was added to fish feed. The proliferation of microbes in the feed was significantly inhibited by the transgenic rice bran. Furthermore, the mortality of tilapias challenged with Aeromonas hydrophila was also significantly reduced when they were fed with the transgenic bran expressing penaeidin3-2. Their intestinal flora changed when the fish were fed with rice bran expressing the introduced gene for a relatively long period. Fish that survived the challenge had a more intact midgut structure. These results indicate that penaeidin3-2 expressed in rice plants may possess the ability to modulate the intestinal bacterial community and protect the gut structure from damage.
学科主题	Fisheries
收录类别	SCI
语种	英语
WOS记录号	WOS:000340571600003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24241]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Qingdao 266071, Peoples R China 2.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Mei,Wang, Baojie,Jiang, Keyong,et al. Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2014,45(3):269-278.
APA	Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,Gong, Kui.,Sun, Shujuan.,...&Fu, Yaping.(2014).Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,45(3),269-278.
MLA	Liu, Mei,et al."Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 45.3(2014):269-278.