Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite

	Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite
	Luo Zuoyong 1,2; Wang Baojie 1; Liu Mei 1; Jiang Keyong 1; Liu Mingxing 1; Wang Lei 1
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2014-07-01
卷号	32 期号:4 页码:749-763
关键词	sea cucumber Apostichopus japonicus Selenka vitamin C nitrite stress antioxidant enzyme reactive oxygen species (ROS) response surface methodology (RSM)
通讯作者	Wang, L (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China.
英文摘要	Different amounts of vitamin C were added to diets fed to juveniles (2.5 +/- 0.15 g) of sea cucumber Apostichopus japonicus (Selenka) in an attempt to reduce the stress response of specimens exposed to nitrite stress. A commercial feed was used as the control diet and three experimental diets were made by supplementing 1 000, 1 500, or 2 000 mg vitamin C/kg diet to control diet separately in a 45-day experiment. Sea cucumbers were exposed to three different levels (0.5, 1.0, and 1.5 mg/L) of nitrite stress for 4, 8, and 12 h at four time intervals (0, 15, 30, and 45 d). Growth of the animals was recorded during the experiment. Reactive oxygen species (ROS) (i.e. hydroxyl free radical (-OH), malondialdehyde (MDA) and total antioxidant capacity (T-AOC)) and antioxidant enzyme activities (i. e., superoxide dismutase (SOD) and catalase (CAT)) were measured. Response surface methodology (RSM) was used to analyze the effect of multiple factors on ROS indices and enzyme activities. Weight gain (WG) and special growth rate (SGR) of vitamin C supplementation groups were significantly higher than those of control group (P<0.05). The levels of -OH and MDA increased under exposure time extending and nitrite concentration increasing, whereas T-AOC level decreased. SOD and CAT activities increased at 4 h and 8 h and decreased at 12 h. During the days in which the animal consumed experimental diets, the levels of -OH and MDA decreased and that of T-AOC increased. This result suggests that diets containing vitamin C could reduce the nitrite stress response in the animals and increase their antioxidant capacity. The multifactor regression equation of growth performance, ROS indices, and duration of feeding results suggest that vitamin C supplementation of 1 400-2 000 mg/kg diet for 29-35 days could reduce effectively the effects of nitrite exposure.
学科主题	Marine & Freshwater Biology; Oceanography
收录类别	SCI
语种	英语
WOS记录号	WOS:000339046200003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24207]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Luo Zuoyong,Wang Baojie,Liu Mei,et al. Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(4):749-763.
APA	Luo Zuoyong,Wang Baojie,Liu Mei,Jiang Keyong,Liu Mingxing,&Wang Lei.(2014).Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(4),749-763.
MLA	Luo Zuoyong,et al."Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.4(2014):749-763.