A Rare Phaeodactylum tricornutum Cruciform Morphotype: Culture Conditions, Transformation and Unique Fatty Acid Characteristics

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	A Rare Phaeodactylum tricornutum Cruciform Morphotype: Culture Conditions, Transformation and Unique Fatty Acid Characteristics
	He, Liyan 1,2; Han, Xiaotian 1; Yu, Zhiming1
刊名	PLOS ONE
	2014-04-07
卷号	9 期号:4 页码:e93922
通讯作者	Yu, ZM (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China.
英文摘要	A rare Phaeodactylum tricornutum cruciform morphotype was obtained and stabilized with a proportion of more than 31.3% in L1 medium and is reported for the first time. Long-term culture and observation showed that the cruciform morphotype was capable of transforming to the oval form following the degeneration of arms by two processes. After three months of culture, four morphotypes existed in a relatively stable proportion in culture for six months (10.5% for oval, 11.3% for fusiform, 37.2% for triradiate and 41.0% for cruciform). Low temperature was particularly beneficial for cruciform cell formation. As the culture temperature decreased from 25 degrees C to 10 degrees C, the percentage of the cruciform morphotype increased from 39.1% to 55.3% approximately. The abundant cruciform cells endowed this strain with unique fatty acid characteristics. The strain cultured at 15 degrees C showed both maximum content of neutral lipid in a single cell and total yield. The maximum content of fatty acid methyl esters was C16: 1 for Phaeodactylum tricornutum cultured at four temperatures (43.82% to 50.82%), followed by C16: 0 (20.47% to 22.65%). Unique fatty acid composition endowed this strain with excellent quality for biodiesel production.
学科主题	Science & Technology - Other Topics
收录类别	SCI
语种	英语
WOS记录号	WOS:000334159800071
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/23987]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He, Liyan,Han, Xiaotian,Yu, Zhiming. A Rare Phaeodactylum tricornutum Cruciform Morphotype: Culture Conditions, Transformation and Unique Fatty Acid Characteristics[J]. PLOS ONE,2014,9(4):e93922.
APA	He, Liyan,Han, Xiaotian,&Yu, Zhiming.(2014).A Rare Phaeodactylum tricornutum Cruciform Morphotype: Culture Conditions, Transformation and Unique Fatty Acid Characteristics.PLOS ONE,9(4),e93922.
MLA	He, Liyan,et al."A Rare Phaeodactylum tricornutum Cruciform Morphotype: Culture Conditions, Transformation and Unique Fatty Acid Characteristics".PLOS ONE 9.4(2014):e93922.