Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry

	Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry
	Zheng, Yu ; Yang, Cheng ; Yang, Fan ; Yang,Xiurong
刊名	analytical chemistry
	2014
卷号	86 期号:8 页码:3849-3855
关键词	CONFORMATIONAL-CHANGES PROTEIN ADSORPTION MULTILAYER FILMS IMMOBILIZATION HYBRIDIZATION BIOSENSOR CYSTEINE SPECTROSCOPY ANTIBODY BINDING
通讯作者	yang,f
英文摘要	the real-time conformational changes of cytosine (c)-rich ssdna oligonucleotides upon binding with silver ions (ag+) were studied using dual polarization interferometry (dpi). upon the addition of ag+, ag+ selectively bound to cytosine cytosine mismatches and formed c-ag+-c complexes, inducing change of the structure of the c-rich ssdna from random coil conformation to duplex conformation, whereas the control ssdna without cytosine-cytosine mismatches had no such signal, which was consistent with circular dichroism (cd) characterization. the conformational change of dna was reflected on the changes of the mass, thickness, and density values resolved by dpi. the calibration curves showed that as the concentration of ag+ increased from 10 nm to 8 mu m, the thickness and mass values increased linearly while the density values decreased linearly. other metal ions such as k+, ca2+, na+, mg2+, zn2+, mn2+, ni2+, and pb2+ did not interfere with the interaction between ag+ and c-rich ssdna, indicating that this method had a good selectivity. the practical application of this biosensor was also investigated in real samples such as drinking water. besides, cysteine could specifically capture ag+ from c-ag+-c complexes and transformed the structure of the c-rich dna back from rigid double-stranded conformation to random coil conformation, which allowed cysteine to be detected selectively as well. it is expected that this biosensing strategy may be utilized to study the interaction of dna with other molecules.
收录类别	SCI
语种	英语
WOS记录号	WOS:000334658000026
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/52385]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zheng, Yu,Yang, Cheng,Yang, Fan,et al. Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry[J]. analytical chemistry,2014,86(8):3849-3855.
APA	Zheng, Yu,Yang, Cheng,Yang, Fan,&Yang,Xiurong.(2014).Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry.analytical chemistry,86(8),3849-3855.
MLA	Zheng, Yu,et al."Real-Time Study of Interactions between Cytosine-Cytosine Pairs in DNA Oligonucleotides and Silver Ions Using Dual Polarization Interferometry".analytical chemistry 86.8(2014):3849-3855.