Nucleic Acid-Regulated Perylene Probe-Induced Gold Nanoparticle Aggregation: A New Strategy for Colorimetric Sensing of Alkaline Phosphatase Activity and Inhibitor Screening

	Nucleic Acid-Regulated Perylene Probe-Induced Gold Nanoparticle Aggregation: A New Strategy for Colorimetric Sensing of Alkaline Phosphatase Activity and Inhibitor Screening
	Jiao, Huping ; Chen, Jian ; Li, Wenying ; Wang,Fangyuan ; Zhou,Huipeng ; Li,Yongxin ; Yu,Cong
刊名	acs applied materials & interfaces
	2014
卷号	6 期号:3 页码:1979-1985
关键词	TURN-ON DETECTION SERUM DNA ASSAY AMPLIFICATION RESOLUTION MOLECULES PROTEIN SURFACE
通讯作者	yu,c
英文摘要	a positively charged perylene probe (probe 1) could induce aggregation of the gold nanoparticles (au nps). as a result, significant assay solution color changes were observed. a duplex dna (dna-1) could induce aggregation of the perylene probe. it was observed that dna-1 could efficiently regulate the probe 1-induced au np aggregation. when probe 1 and dna-1 were first mixed, dna-1 induced aggregation of the perylene probe. au nps were subsequently added, and no induced aggregation of the au nps was observed. thus the color of the assay solution remained to be red. the assay is quite sensitive; 200 pm dna-1 could cause a clear solution color change. on the basis of this observation, a novel method for the detection of alkaline phosphatase (alp) activity has been demonstrated. our method does not require covalent immobilization of the nucleic acid, or the addition of an excess amount of salt. it is sensitive and convenient.
收录类别	SCI
语种	英语
WOS记录号	WOS:000331493200084
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/52356]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Jiao, Huping,Chen, Jian,Li, Wenying,et al. Nucleic Acid-Regulated Perylene Probe-Induced Gold Nanoparticle Aggregation: A New Strategy for Colorimetric Sensing of Alkaline Phosphatase Activity and Inhibitor Screening[J]. acs applied materials & interfaces,2014,6(3):1979-1985.
APA	Jiao, Huping.,Chen, Jian.,Li, Wenying.,Wang,Fangyuan.,Zhou,Huipeng.,...&Yu,Cong.(2014).Nucleic Acid-Regulated Perylene Probe-Induced Gold Nanoparticle Aggregation: A New Strategy for Colorimetric Sensing of Alkaline Phosphatase Activity and Inhibitor Screening.acs applied materials & interfaces,6(3),1979-1985.
MLA	Jiao, Huping,et al."Nucleic Acid-Regulated Perylene Probe-Induced Gold Nanoparticle Aggregation: A New Strategy for Colorimetric Sensing of Alkaline Phosphatase Activity and Inhibitor Screening".acs applied materials & interfaces 6.3(2014):1979-1985.