Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture | |
Sun, Lijing1; Xiong, Zhidong1,2; Zhou, Weiqing1,3; Liu, Ruina1; Yan, Xingran3; Li, Juan3; An, Wenqi4; Yuan, Guoqiang4; Ma, Guanghui1,3; Su, Zhiguo1,3 | |
刊名 | BIOCHEMICAL ENGINEERING JOURNAL |
2015-04-15 | |
卷号 | 96期号:APR页码:46-54 |
关键词 | Konjac glucomannan Microcarriers Animal cell culture Anion exchange capacity Large-scale cultivation Glucose |
ISSN号 | 1369-703X |
通讯作者 | Su, ZG (reprint author), Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China. |
英文摘要 | A new type of microcarrier was prepared for anchorage-dependent animal cell culture. Similar to the commercial product Cytodex 1, the newcomer is also spherical in micro-size range with ionic group DEAE, but the matrix is konjac glucomannan (KGM) from a natural plant. The optimal anion exchange capacity (AEC) was found to be 2.1 to 2.4 mmol/g dry microspheres. Five cell lines were cultured in static mode with the KGM microcarriers, including Vero, CHO-K1, MDCK, Wish and L929 cells. These cells adhered well and grew to high cell density comparable with or even better than those did on Cytodex 1. A further comparison was made with Vero cell culture in spinner mode. It was found that Vero cells achieved more than 2 x 10(6) cells/ml, and showed faster cell adhesion and higher specific growth rate on the KGM microcarriers than on Cytodex 1. In 5 L bioreactor perfusion culture, Vero cells grew above 7.0 x 10(6) cells/ml and the rabies virus titer was about 7.7 IgLD(50)/ml, compared to Cytodex 1 in the same culture condition. The results suggest that the new type of microcarrier could be a promising candidate for various cell cultures. (C) 2014 Elsevier B.V. All rights reserved. |
学科主题 | Biotechnology & Applied Microbiology ; Engineering |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
关键词[WOS] | VERO CELLS ; RABIES VIRUS ; JAPANESE ENCEPHALITIS ; MICROROUGH SURFACE ; STEM-CELLS ; IN-VITRO ; GROWTH ; ATTACHMENT ; BIOREACTOR ; HYDROGEL |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000350776500007 |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/13779] |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Engn Res Ctr Biotechnol Beijing, Beijing 100190, Peoples R China 4.Hualan Biol Bacterin Co Ltd, Xinxiang 453003, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Lijing,Xiong, Zhidong,Zhou, Weiqing,et al. Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture[J]. BIOCHEMICAL ENGINEERING JOURNAL,2015,96(APR):46-54. |
APA | Sun, Lijing.,Xiong, Zhidong.,Zhou, Weiqing.,Liu, Ruina.,Yan, Xingran.,...&Su, Zhiguo.(2015).Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture.BIOCHEMICAL ENGINEERING JOURNAL,96(APR),46-54. |
MLA | Sun, Lijing,et al."Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture".BIOCHEMICAL ENGINEERING JOURNAL 96.APR(2015):46-54. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论