Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 研究所（批量导入）

	Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization
	Liu, Ying 1; Guo, Chen 2; Sun, Xi-Tong 2; Liu, Chun-Zhao 1
刊名	BIORESOURCE TECHNOLOGY
	2013-08-01
卷号	142 期号:0 页码:415-419
关键词	Yarrowia lipolytica lipase Bioimprinting Immobilization Resolution 2-Octanol
英文摘要	Yarrowia lipolytica lipase (YLL) demonstrated an (R)-enantiopreference for efficient resolution of (R,S)-2-octanol. The activity, enantioselectivity, the ratio of substrate to enzyme, acetaldehyde tolerance, and operational stability of YLL were improved by an integrated strategy of interfacial activation, bioimprinting, and immobilization. In comparison with the control, both the enzymatic activity and enantioselectivity increased by a factor of 8.85 and 2.75 by the integrated strategy, respectively. Fifty-one percentage of conversion with 220 of enantioselectivity was obtained using the immobilized YLL prepared by the integrated strategy at a ratio of 104 of substrate to enzyme loaded. The immobilized YLL retained 97% of its initial activity without a decrease in enantioselectivity after 10 successive reuse cycles. Together these results will result in a promising strategy with the YYL for efficient resolution of (R,S)-2-octanol in practice. (C) 2013 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	POLYUNSATURATED FATTY-ACIDS ; MACROPOROUS RESIN ; ACETALDEHYDE ; ADSORPTION ; STABILIZATION ; PROTEINS ; ENZYMES ; BINDING
收录类别	SCI
语种	英语
WOS记录号	WOS:000322292800055
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/13768]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ying,Guo, Chen,Sun, Xi-Tong,et al. Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization[J]. BIORESOURCE TECHNOLOGY,2013,142(0):415-419.
APA	Liu, Ying,Guo, Chen,Sun, Xi-Tong,&Liu, Chun-Zhao.(2013).Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization.BIORESOURCE TECHNOLOGY,142(0),415-419.
MLA	Liu, Ying,et al."Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization".BIORESOURCE TECHNOLOGY 142.0(2013):415-419.