Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 研究所（批量导入）

	Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI
	Feng, Xiaoyi 1,2; Gao, Fei 1; Qin, Peiyong 2; Ma, Guanghui 1; Su, Zhiguo 1; Ge, Jia1 ; Wang, Ping 3,4; Zhang, Songping 1
刊名	ANALYTICAL CHEMISTRY
	2013-02-19
卷号	85 期号:4 页码:2370-2376
英文摘要	We report in this work real time characterization of enzyme coenzyme binding by using surface plasmon resonance (SPR) and dual polarization interferometry (DPI) analyses. Results showed that diaphorase (DP) and lactate dehydrogenases (LDH) had distinct binding selectivity and preference over reduced and oxidized states of coenzyme NAD(H). On the basis of that, DP and LDH were chosen as indicator enzymes to distinguish the specific state of surface-bound NAD(H). The transformation between NADH and NAD+ during enzyme-catalyzed redox reactions was therefore transduced into variation in interaction signals as indicated via the binding status of the indicator enzymes as detected with both SPR and DPI. This real time molecule-specific detection strategy revealed quick and direct reflection of the state and reactivity of the coenzyme, promising a unique way of precise molecular interaction analysis.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	DUAL-POLARIZATION INTERFEROMETRY ; NICOTINAMIDE-ADENINE DINUCLEOTIDE ; PROTEIN ADSORPTION ; AFFINITY CHROMATOGRAPHY ; RESONANCE SPECTROSCOPY ; COFACTOR REGENERATION ; DNA IMMOBILIZATION ; SURFACE ; ELLIPSOMETRY ; DERIVATIVES
收录类别	SCI
语种	英语
WOS记录号	WOS:000315326900058
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/13726]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Univ Chem Technol, Coll Life Sci, Beijing 100029, Peoples R China 3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA 4.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
推荐引用方式 GB/T 7714	Feng, Xiaoyi,Gao, Fei,Qin, Peiyong,et al. Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI[J]. ANALYTICAL CHEMISTRY,2013,85(4):2370-2376.
APA	Feng, Xiaoyi.,Gao, Fei.,Qin, Peiyong.,Ma, Guanghui.,Su, Zhiguo.,...&Zhang, Songping.(2013).Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI.ANALYTICAL CHEMISTRY,85(4),2370-2376.
MLA	Feng, Xiaoyi,et al."Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI".ANALYTICAL CHEMISTRY 85.4(2013):2370-2376.