Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins

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	Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins
	Liu, Boyan 1,2; Ouyang, Jie 2; Yuan, Xiaofan 1; Wang, Liwei1 ; Zhao, Bing 1
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2013-10-15
卷号	937 期号:0 页码:84-90
关键词	Macroporous resin Phenylethanoid glycosides Echinacoside Acteoside Cistanche deserticola Y. C. Ma Separation
英文摘要	A simple and efficient chromatographic method for large-scale preparative separation of phenylethanoid glycosides (mainly contain echinacoside and acteoside) from Cistanche deserticola was developed. The adsorption properties of eight macroporous resins were evaluated. Three selected resins were further screened depending on the adsorption kinetics curves, in which HPD300 resin showed the best separation efficiency. The adsorption isotherm data on HPD300 resin were fitted to the Freundlich equation in certain concentration range. The dynamic adsorption and desorption experiments were carried out on columns packed with HPD300 resin to optimize the separation process. The breakthrough curves showed that acteoside had a higher affinity to the resin than echinacoside. The contents of echinacoside and acteoside in the product increased from 1.79% and 1.43% in the crude extracts to 16.66% and 15.17%, with recovery yields of 80.41% and 90.17%, respectively. The purity of total phenylethanoid glycosides in the product was 76.58%. (c) 2013 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	COUNTER-CURRENT CHROMATOGRAPHY ; DIODE-ARRAY DETECTION ; COMPETITIVE ADSORPTION ; MASS-SPECTROMETRY ; TUBULOSA ; CONSTITUENTS ; PURIFICATION ; OLIGOGLYCOSIDES ; NITROPHENOL ; FLAVONOIDS
收录类别	SCI
语种	英语
WOS记录号	WOS:000325040400012
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/13594]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Being Forestry Univ, Coll Biol Sci & Biotechnol, Dept Food Sci & Engn, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Liu, Boyan,Ouyang, Jie,Yuan, Xiaofan,et al. Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,937(0):84-90.
APA	Liu, Boyan,Ouyang, Jie,Yuan, Xiaofan,Wang, Liwei,&Zhao, Bing.(2013).Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,937(0),84-90.
MLA	Liu, Boyan,et al."Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 937.0(2013):84-90.