Codelivery of mTERT siRNA and paclitaxel by chitosan-based nanoparticles promoted synergistic tumor suppression

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	Codelivery of mTERT siRNA and paclitaxel by chitosan-based nanoparticles promoted synergistic tumor suppression
	Wei, Wei 1; Lv, Pi-Ping 1,2; Chen, Xiao-Ming 1,2; Yue, Zhan-Guo 1,2; Fu, Qiang 1,3; Liu, Shi-Ying 1,2; Yue, Hua 1; Ma, Guang-Hui 1
刊名	BIOMATERIALS
	2013-05-01
卷号	34 期号:15 页码:3912-3923
关键词	siRNA Paclitaxel Drug delivery Chitosan Nanoparticles
英文摘要	Clinical applications of siRNA are being hindered by poor intracellular uptake and enzymatic degradation. To address these problems, we devised an oral delivery system for telomerase reverse transcriptase siRNA using N-((2-hydroxy-3-trimethylammonium) propyl) chitosan chloride (HTCC) nanoparticles (HNP). Both the porous structure and the positive charge of HNP facilitated siRNA encapsulation. The outer coating of HTCC not only protected siRNA from enzymatic degradation, but also improved siRNA permeability in intestine tract. In vivo and in vitro experiments proved that HNP could effectively deliver siRNA to lesion site and further into tumor cells. On the basis of confirming the antitumor activity of HNP:siRNA, we continued to encapsulate a hydrophobic chemotherapeutic drug-paclitaxel (PTX) into HNP to form a "two-in-one" nano-complex (HNP:siRNA/PTX). We demonstrated that HNP:siRNA/PTX could simultaneously ferry siRNA and PTX into tumor cells and increase drug concentration, which, in particular, was much more effective in tumor suppression than that of traditional cocktail therapy. These results suggested that the HNP, as a powerful delivery system for both siRNA and chemotherapeutic drug, would have a far-reaching application in human cancer therapy. (C) 2013 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	CANCER-CELLS ; CO-DELIVERY ; QUATERNIZED CHITOSAN ; TELOMERASE ACTIVITY ; IN-VIVO ; GROWTH ; THERAPEUTICS ; THERAPY ; SYSTEM ; TARGET
收录类别	SCI
语种	英语
WOS记录号	WOS:000317168700018
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/13438]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shandong Univ, Life Sci Coll, Key Lab Cell & Dev Biol, Jinan 250100, Peoples R China
推荐引用方式 GB/T 7714	Wei, Wei,Lv, Pi-Ping,Chen, Xiao-Ming,et al. Codelivery of mTERT siRNA and paclitaxel by chitosan-based nanoparticles promoted synergistic tumor suppression[J]. BIOMATERIALS,2013,34(15):3912-3923.
APA	Wei, Wei.,Lv, Pi-Ping.,Chen, Xiao-Ming.,Yue, Zhan-Guo.,Fu, Qiang.,...&Ma, Guang-Hui.(2013).Codelivery of mTERT siRNA and paclitaxel by chitosan-based nanoparticles promoted synergistic tumor suppression.BIOMATERIALS,34(15),3912-3923.
MLA	Wei, Wei,et al."Codelivery of mTERT siRNA and paclitaxel by chitosan-based nanoparticles promoted synergistic tumor suppression".BIOMATERIALS 34.15(2013):3912-3923.