Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants

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	Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants
	Zhao, Y.1,3; Stiles, A. R.1; Saxena, P. K.2; Liu, C. Z.1
刊名	BIOLOGIA PLANTARUM
	2013-03-01
卷号	57 期号:1 页码:189-192
关键词	auxin cytokinin gibberellic acid polyphenol oxidase
ISSN号	0006-3134
英文摘要	An efficient in vitro plant regeneration system has been developed using dark preincubated leaf explants of Rhodiola crenulata, a traditional Tibetan medicinal plant. The leaf explants, preincubated in the dark for 5 d, developed an average of 9.1 shoots per explant on a medium containing 15 mu M N (6)-benzyladenine (BA) and 2.5 mu M gibberellic acid (GA(3)). The biochemical mechanism underlying dark-induced shoot organogenesis was investigated by measuring polyphenol oxidase (PPO) activity. Dark preincubation significantly reduced PPO activity in leaf explants during the initial period of shoot organogenesis and reduced browning compared to explants cultured in the light. Up to 88.4 % of the regenerated shoots formed roots and developed into complete plantlets on a medium containing 5 mu M indoleacetic acid (IAA) within 25 d. Approximately 82 % of the regenerated plantlets survived transplantation and grew vigorously in the greenhouse.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	IN-VITRO ; SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; TISSUE-CULTURES ; GROWTH
收录类别	SCI
语种	英语
WOS记录号	WOS:000318647800029
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/13316]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Y.,Stiles, A. R.,Saxena, P. K.,et al. Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants[J]. BIOLOGIA PLANTARUM,2013,57(1):189-192.
APA	Zhao, Y.,Stiles, A. R.,Saxena, P. K.,&Liu, C. Z..(2013).Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants.BIOLOGIA PLANTARUM,57(1),189-192.
MLA	Zhao, Y.,et al."Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants".BIOLOGIA PLANTARUM 57.1(2013):189-192.