Preparation and characterization of a thermoresponsive gigaporous medium for high-speed protein chromatography | |
Qu, J. B.; Chen, Y. L.; Huan, G. S.; Zhou, W. Q.; Liu, J. G.; Zhu, H.; Zhang, X. Y. | |
刊名 | Analytica Chimica Acta
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2015 | |
期号 | 0页码:617-624 |
关键词 | Gigaporous polystyrene microspheres Thermoresponsive polymer brushes Protein chromatography N-isopropylacrylamide Atom transfer radical polymerization POLYSTYRENE MICROSPHERES AQUEOUS CHROMATOGRAPHY LIQUID-CHROMATOGRAPHY STATIONARY-PHASE SEPARATION POLYMERS SILICA BEADS |
其他题名 | Anal. Chim. Acta |
中文摘要 | A high-speed thermoresponsive medium was developed by grafting poly(N-isopropylacrylamide-co-butyl methacrylate) (P(NIPAM-co-BMA)) brushes onto gigaporous polystyrene (PS) microspheres via surface-initiated atom transfer radical polymerization (ATRP) technique, which has strong mechanical strength, good chemical stability and high mass transfer rate for biomacromolecules. The gigaporous structure, surface chemical composition, static protein adsorption, and thermoresponsive chromatographic properties of prepared medium (PS-P(NIPAM-co-BMA)) were characterized in detail. Results showed that the PS microspheres were successfully grafted with P(NIPAM-co-BMA) brushes and that the gigaporous structure was robustly maintained. After grafting, the nonspecific adsorption of proteins on PS microspheres was greatly reduced. A column packed with PS-P(NIPAM-co-BMA) exhibited low backpressure and significant thermo-responsibility. By simply changing the column temperature, it was able to separate three model proteins at the mobile phase velocity up to 2167 cm h(-1). In conclusion, the thermoresponsive polymer brushes grafted gigaporous PS microspheres prepared by ATRP are very promising in 'green' high-speed preparative protein chromatography. (C) 2014 Elsevier B.V. All rights reserved. |
收录类别 | SCI |
原文出处 | |
语种 | 英语 |
公开日期 | 2015-04-01 |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/11816] ![]() |
专题 | 过程工程研究所_研究所(批量导入) |
推荐引用方式 GB/T 7714 | Qu, J. B.,Chen, Y. L.,Huan, G. S.,et al. Preparation and characterization of a thermoresponsive gigaporous medium for high-speed protein chromatography[J]. Analytica Chimica Acta,2015(0):617-624. |
APA | Qu, J. B..,Chen, Y. L..,Huan, G. S..,Zhou, W. Q..,Liu, J. G..,...&Zhang, X. Y..(2015).Preparation and characterization of a thermoresponsive gigaporous medium for high-speed protein chromatography.Analytica Chimica Acta(0),617-624. |
MLA | Qu, J. B.,et al."Preparation and characterization of a thermoresponsive gigaporous medium for high-speed protein chromatography".Analytica Chimica Acta .0(2015):617-624. |
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