Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core

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	Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core
	Cui, Jian Dong 1,2,3; Li, Lian Lian 1; Zhao, Ya Min 1
刊名	INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
	2014-10-22
卷号	53 期号:42 页码:16176-16182
关键词	PHENYLALANINE AMMONIA-LYASE IMMOBILIZATION STABILIZATION PHENYLKETONURIA CLEAS MICROCAPSULES BIOCATALYSTS REPLACEMENT STRATEGIES STABILITY
ISSN号	0888-5885
其他题名	Ind. Eng. Chem. Res.
中文摘要	With the aim of providing a highly stable and easy recyclable biocatalyst, we prepared a new biocatalyst formulation based on cross-linked enzyme aggregate (CLEA) technology by the physical adsorption of cross-linked phenylalanine ammonia lyase on a highly porous microspherical silica gel solid support (S-CLEAs). The main factors in the preparation of S-CLEAs were optimized by response surface methodology (RSM). Analysis by scanning electron microscopy (SEM), FT-IR spectroscopy, and confocal laser scanning microscopy (CLSM) indicated that the CLEAs were successfully immobilized on the crude-pored microspherical silica gel. Compared to standard CLEAs, the S-CLEAs can be easily separated from the reaction mixture by natural precipitation without centrifugation and filtration treatments. Although the S-CLEAs revealed an excellent stability of the enzyme against high temperature and denaturants, the volumetric activity of the S-CLEAs decreased because of the presence of silica gel. In a word, the S-CLEA technology in this study might open an attractive route toward effectively improving the catalytic properties of traditional CLEAs.
英文摘要	With the aim of providing a highly stable and easy recyclable biocatalyst, we prepared a new biocatalyst formulation based on cross-linked enzyme aggregate (CLEA) technology by the physical adsorption of cross-linked phenylalanine ammonia lyase on a highly porous microspherical silica gel solid support (S-CLEAs). The main factors in the preparation of S-CLEAs were optimized by response surface methodology (RSM). Analysis by scanning electron microscopy (SEM), FT-IR spectroscopy, and confocal laser scanning microscopy (CLSM) indicated that the CLEAs were successfully immobilized on the crude-pored microspherical silica gel. Compared to standard CLEAs, the S-CLEAs can be easily separated from the reaction mixture by natural precipitation without centrifugation and filtration treatments. Although the S-CLEAs revealed an excellent stability of the enzyme against high temperature and denaturants, the volumetric activity of the S-CLEAs decreased because of the presence of silica gel. In a word, the S-CLEA technology in this study might open an attractive route toward effectively improving the catalytic properties of traditional CLEAs.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Chemical
研究领域[WOS]	Engineering
关键词[WOS]	PHENYLALANINE AMMONIA-LYASE ; IMMOBILIZATION ; STABILIZATION ; PHENYLKETONURIA ; CLEAS ; MICROCAPSULES ; BIOCATALYSTS ; REPLACEMENT ; STRATEGIES ; STABILITY
收录类别	SCI
原文出处	://WOS:000343687400008
语种	英语
WOS记录号	WOS:000343687400008
公开日期	2014-12-01
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/11668]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang 050000, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Cui, Jian Dong,Li, Lian Lian,Zhao, Ya Min. Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2014,53(42):16176-16182.
APA	Cui, Jian Dong,Li, Lian Lian,&Zhao, Ya Min.(2014).Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,53(42),16176-16182.
MLA	Cui, Jian Dong,et al."Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 53.42(2014):16176-16182.