Model of EF4-induced ribosomal state transitions and mRNA translocation

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	Model of EF4-induced ribosomal state transitions and mRNA translocation
	Xie, P
刊名	PHYSICAL BIOLOGY
	2014
卷号	11 期号:4
关键词	ribosome LepA translation EF-G puromycin molecular machine
ISSN号	1478-3967
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Key Lab Soft Matter Phys, Inst Phys, Beijing 100190, Peoples R China.
中文摘要	EF4, a highly conserved protein present in bacteria, mitochondria and chloroplasts, can bind to both the posttranslocation and pretranslocation ribosomal complexes. When binding to the posttranslocation state, it catalyzes backward translocation to a pretranslocation state. When binding to the pretranslocation state, it catalyzes transition to another pretranslocation state that is similar and possibly identical to that resulting from the posttranslocation state bound by EF4, and competes with EF-G to regulate the elongation cycle. However, the molecular mechanism on how EF4 induces state transitions and mRNA translocation remains unclear. Here, we present both the model for state transitions induced by EF4 binding to the posttranslocation state and that by EF4 binding to the pretranslocation state, based on which we study the kinetics of EF4-induced state transitions and mRNA translocation, giving quantitative explanations of the available experimental data. Moreover, we present some predicted results on state transitions and mRNA translocation induced by EF4 binding to the pretranslocation state complexed with the mRNA containing a duplex region.
资助信息	National Natural Science Foundation of China [11374352]
语种	英语
公开日期	2015-04-14
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/59447]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Xie, P. Model of EF4-induced ribosomal state transitions and mRNA translocation[J]. PHYSICAL BIOLOGY,2014,11(4).
APA	Xie, P.(2014).Model of EF4-induced ribosomal state transitions and mRNA translocation.PHYSICAL BIOLOGY,11(4).
MLA	Xie, P."Model of EF4-induced ribosomal state transitions and mRNA translocation".PHYSICAL BIOLOGY 11.4(2014).