Using Xe as a heavy atom for phase determination of protein trichosanthin structure

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Using Xe as a heavy atom for phase determination of protein trichosanthin structure
	Chen, YX ; Li, MJ ; Yu, F ; Liu, K ; Xu, CY ; Sun, B ; Zhou, H ; Xu, Q ; He, JH
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2014
卷号	25 期号:3
关键词	RIBOSOME-INACTIVATING PROTEIN ANGSTROM RESOLUTION CRYSTAL-STRUCTURE BINDING XENON ALPHA CHANNELS REVEALS HISTORY COMPLEX
ISSN号	1001-8042
通讯作者	liminjun@sinap.ac.cn ; hejh@sinap.ac.cn
英文摘要	Under gas pressures of 1-3 MPa, xenon can be bound to discrete sites in hydrophobic cavities of protein, so as to use Xe as a heavy atom for determining phases in protein crystallography. Using single anomalous scattering diffraction method, we demonstrate that an interpretable electron density map can be obtained for protein trichosanthin from a single xenon derivative. We found, for the first time, a pre-existing hydrophobic cavity just under the protein surface of trichosanthin.
收录类别	SCI
语种	英语
WOS记录号	WOS:000339366300013
公开日期	2015-03-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/14546]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Chen, YX,Li, MJ,Yu, F,et al. Using Xe as a heavy atom for phase determination of protein trichosanthin structure[J]. NUCLEAR SCIENCE AND TECHNIQUES,2014,25(3).
APA	Chen, YX.,Li, MJ.,Yu, F.,Liu, K.,Xu, CY.,...&He, JH.(2014).Using Xe as a heavy atom for phase determination of protein trichosanthin structure.NUCLEAR SCIENCE AND TECHNIQUES,25(3).
MLA	Chen, YX,et al."Using Xe as a heavy atom for phase determination of protein trichosanthin structure".NUCLEAR SCIENCE AND TECHNIQUES 25.3(2014).