Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging

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	Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging
	Zhu, L ; Wang, K ; Cui, J ; Liu, H ; Bu, XL ; Ma, HL ; Wang, WZ ; Gong, H ; Lausted, C ; Hood, L ; Yang, G ; Hu, ZY
刊名	ANALYTICAL CHEMISTRY
	2014
卷号	86 期号:17 页码:8857—8864
关键词	TRANSFERRIN RECEPTOR REJECTION ANTIGENS PROTEOMIC ANALYSIS MEMBRANE-VESICLES HIGH-THROUGHPUT B-LYMPHOCYTES CELL MICROVESICLES CANCER METASTASIS
ISSN号	0003-2700
通讯作者	yangguang9002@163.com ; huzy@nanoctr.cn
英文摘要	Exosomes are endosome-derived membrane vesicles carrying proteins and nucleic adds that are involved in cellular functions such as intercellular communication, protein and RNA secretion, and antigen presentation. Therefore, exosomes serve as potential biomarkers for many diseases including cancer. Because exosomes are difficult to enrich or purify from biofluids, quantification of exosomes is tedious and inaccurate. Here, we present a real-time, label-free, and quantitative method to detect and characterize tumor-derived exosomes without enrichment or purification. Utilizing surface plasmon resonance imaging (SPRi) in combination with antibody microarrays specific to the extracellular domains of exosome membrane proteins, exosomes in tumor cell culture medium can be quantitatively detected. We found a positive correlation between the metastatic potential of tumor cell lines and exosome secretion. This method provides an easy, efficient, and novel way to detect exosome secretion and thus an avenue toward the diagnosis and prognosis prediction of cancer.
收录类别	SCI
语种	英语
WOS记录号	WOS:000341229200050
公开日期	2015-03-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/14211]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zhu, L,Wang, K,Cui, J,et al. Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging[J]. ANALYTICAL CHEMISTRY,2014,86(17):8857—8864.
APA	Zhu, L.,Wang, K.,Cui, J.,Liu, H.,Bu, XL.,...&Hu, ZY.(2014).Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging.ANALYTICAL CHEMISTRY,86(17),8857—8864.
MLA	Zhu, L,et al."Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging".ANALYTICAL CHEMISTRY 86.17(2014):8857—8864.