Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 土壤环境科学实验室

	Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis
	Ye, Jun ; Chang, Yue ; Yan, Yu ; Xiong, Jie ; Xue, Xi-Mei ; Yuan, Dongxia ; Sun, Guo-Xin ; Zhu, Yong-Guan ; Miao, Wei
刊名	AQUATIC TOXICOLOGY
	2014
卷号	149 期号:1 页码:50-57
关键词	Tetrahymena pyriformis Protozoan Arsenic Methyltransferase Methylarsine Heavy metal
ISSN号	0166-445X
中文摘要	Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coli; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved.
WOS记录号	WOS:000334989300007
公开日期	2015-03-26
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9639]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Ye, Jun,Chang, Yue,Yan, Yu,et al. Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis[J]. AQUATIC TOXICOLOGY,2014,149(1):50-57.
APA	Ye, Jun.,Chang, Yue.,Yan, Yu.,Xiong, Jie.,Xue, Xi-Mei.,...&Miao, Wei.(2014).Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis.AQUATIC TOXICOLOGY,149(1),50-57.
MLA	Ye, Jun,et al."Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis".AQUATIC TOXICOLOGY 149.1(2014):50-57.