Application of the SOS/umu test and high-content in vitro micronucleus test to determine genotoxicity and cytotoxicity of nine benzothiazoles

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境水质学国家重点实验室

	Application of the SOS/umu test and high-content in vitro micronucleus test to determine genotoxicity and cytotoxicity of nine benzothiazoles
	Yan Ye ; Jiang Weiwei ; Li Na ; Ma Mei ; Rao Kaifeng ; Wang Zijian
刊名	JOURNAL OF APPLIED TOXICOLOGY
	2014
卷号	34 期号:12 页码:1400-1408
关键词	benzothiazoles SOS umu test High-Content in vitro Micronucleus test genotoxicity cytotoxicity
ISSN号	0260-437X
中文摘要	Benzothiazole and benzothiazole derivatives (BTs) have been detected in various environmental matrices as well as in human beings, but little is currently available regarding their toxicities. In our study, genotoxicities of nine BTs (benzothiazole [BT], 2-chlorobenzothiazole [CBT], 2-bromobenzothiazole [BrBT], 2-fluorobenzothiazole [FBT], 2-methylbenzothiazole [MeBT], 2-mercaptobenzothiazole [MBT], 2-aminobenzothiazole [ABT], 2-hydroxy-benzothiazole [OHBT] and 2-methythiobenzothiazole [MTBT]) are comprehensively evaluated by the SOS/umu test using the bacterial Salmonella typhimurium TA1535/pSK1002 for DNA-damaging effect and the high content in vitro micronucleus test using two human carcinoma cells (MGC-803 and A549) for chromosome-damaging effect. The cytotoxicity of BTs on both bacteria and two human cells was also evaluated. Except for the cytotoxic effect of MBT on MGC-803 and A549, the other tested BTs showed more than 50% cytotoxicity at their highest concentrations in a dose-dependent manner, and their LC50s ranged from 19 (MBT in bacteria) to 270 mg l(-1) (CBT in A549). Activation and inactivation were observed for specific BTs after metabolism. On the other hand, no evidence of genotoxicity was obtained for BT, FBT and MBT, and DNA damage was induced by ABT, OHBT, BrBT and MTBT in MGC-803, by MeBT in A549 and by CBT in both cells. Through quantitative structure-activity relationship analysis, two structure alerts for chemical genotoxicity, including heterocyclic amine and hacceptor-path3-hacceptor are present in ABT and OHBT respectively; however, the underlying mechanisms still need further evaluation. Copyright (c) 2014 John Wiley & Sons, Ltd. Cytotoxicity of BTs in both bacterial Salmonella typhimurium TA1535/pSK1002, MGC-803 and A549 cells was observed in a dose-dependent manner, and activation and inactivation were found for specific BTs after metabolism. Six of nine BTs were found to be genotoxic to either bacteria or cells or both, and structure alerts including heterocyclic amine and hacceptor-path3-hacceptor may contribute to the underlying genotoxic mechanism for 2-Aminobenzothiazole and 2-Hydroxy-benzothiazole respectively.
WOS记录号	WOS:000344146300015
公开日期	2015-03-26
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9499]
专题	生态环境研究中心_环境水质学国家重点实验室
推荐引用方式 GB/T 7714	Yan Ye,Jiang Weiwei,Li Na,et al. Application of the SOS/umu test and high-content in vitro micronucleus test to determine genotoxicity and cytotoxicity of nine benzothiazoles[J]. JOURNAL OF APPLIED TOXICOLOGY,2014,34(12):1400-1408.
APA	Yan Ye,Jiang Weiwei,Li Na,Ma Mei,Rao Kaifeng,&Wang Zijian.(2014).Application of the SOS/umu test and high-content in vitro micronucleus test to determine genotoxicity and cytotoxicity of nine benzothiazoles.JOURNAL OF APPLIED TOXICOLOGY,34(12),1400-1408.
MLA	Yan Ye,et al."Application of the SOS/umu test and high-content in vitro micronucleus test to determine genotoxicity and cytotoxicity of nine benzothiazoles".JOURNAL OF APPLIED TOXICOLOGY 34.12(2014):1400-1408.