Dioxin and Dioxin-Like Compounds Suppress Acetylcholinesterase Activity via Transcriptional Downregulations In Vitro

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Dioxin and Dioxin-Like Compounds Suppress Acetylcholinesterase Activity via Transcriptional Downregulations In Vitro
	Xu, Hai-Ming ; Xie, Heidi Qunhui ; Tao, Wu-Qun ; Zhou, Zhi-Guang ; Li, Shuai-Zhang ; Zhao, Bin
刊名	JOURNAL OF MOLECULAR NEUROSCIENCE
	2014
卷号	53 期号:3 页码:417-423
关键词	Acetylcholinesterase (AChE) Aryl hydrocarbon receptor (AhR) Dioxin-responsive element (DRE) Dioxin Transcriptional regulation Neuron
ISSN号	0895-8696
中文摘要	Recently, acetylcholinesterase (AChE, EC 3.1.1.7) has received increased attention in the field of environmental sciences. Evaluation of the effects of environmental contaminants on AChE enzymatic activity not only can reflect, to some extent, the interference with the nervous system, but also can be used for monitoring pollution. Our previous study showed that 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) suppressed neuronal AChE enzymatic activity via transcriptional downregulations mediated by aryl hydrocarbon receptor. In the present study, the effects of several other dioxin-like compounds (DLCs) on neuronal AChE activity were determined, including 1,2,3,7,8-pentachlorodibenzo-p-dioxin, 2,3,7,8-tetrachlorodibenzofuran, 2,3,4,7,8-pentachlorodibenzofuran, and 2,3,7,8-tetrabromodibenzo-p-dioxin. The results showed that the enzymatic activity of AChE was significantly decreased by approximately 15-30 % after exposure to a certain concentrations of the DLCs, whereas incubating neuronal cell lysates directly with these DLCs did not inhibit AChE enzyme. Subsequent molecular mechanism study showed that these chemicals could decrease ACHE promoter activity, as well as AChE (T) mRNA expression, thereby suggesting the involvements of transcriptional regulation in these effects. These findings on DLCs are similar with those on 2,3,7,8-TCDD, pointing to the possibility that exposure to dioxin and DLCs, which frequently coexist in the contaminated environments, may concurrently interfere with the cholinergic functions via AChE.
WOS记录号	WOS:000339956700018
公开日期	2015-03-24
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9341]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Xu, Hai-Ming,Xie, Heidi Qunhui,Tao, Wu-Qun,et al. Dioxin and Dioxin-Like Compounds Suppress Acetylcholinesterase Activity via Transcriptional Downregulations In Vitro[J]. JOURNAL OF MOLECULAR NEUROSCIENCE,2014,53(3):417-423.
APA	Xu, Hai-Ming,Xie, Heidi Qunhui,Tao, Wu-Qun,Zhou, Zhi-Guang,Li, Shuai-Zhang,&Zhao, Bin.(2014).Dioxin and Dioxin-Like Compounds Suppress Acetylcholinesterase Activity via Transcriptional Downregulations In Vitro.JOURNAL OF MOLECULAR NEUROSCIENCE,53(3),417-423.
MLA	Xu, Hai-Ming,et al."Dioxin and Dioxin-Like Compounds Suppress Acetylcholinesterase Activity via Transcriptional Downregulations In Vitro".JOURNAL OF MOLECULAR NEUROSCIENCE 53.3(2014):417-423.