Detection of lead(II) ions with a DNAzyme and isothermal strand displacement signal amplification

	Detection of lead(II) ions with a DNAzyme and isothermal strand displacement signal amplification
	Li, Wenying ; Yang, Yue ; Chen, Jian ; Zhang,Qingfeng ; Wang,Yan ; Wang,Fangyuan ; Yu,Cong
刊名	biosensors & bioelectronics
	2014
卷号	53 期号:1 页码:245-249
关键词	IN-VITRO SELECTION ELECTROCHEMICAL DETECTION COLORIMETRIC DETECTION AMPLIFIED DETECTION GOLD NANOPARTICLES HIGH-SENSITIVITY HEAVY-METAL PB2+ DEOXYRIBOZYME ELECTRODE
通讯作者	yu,c
英文摘要	a dnazyme based method for the sensitive and selective quantification of lead(ii) ions has been developed. a dnazyme that requires pb2+ for activation was selected. an rna containing dna substrate was cleaved by the dnazyme in the presence of pb2+. the 2',3'-cyclic phosphate of the cleaved 5'-part of the substrate was efficiently removed by exonuclease iii. the remaining part of the single stranded dna (9 or 13 base long) was subsequently used as the primer for the strand displacement amplification reaction (sdar). the method is highly sensitive, 200 pm lead(ii) could be easily detected. a number of interference ions were tested, and the sensor showed good selectivity. underground water samples were also tested, which demonstrated the feasibility of the current approach for real sample applications. it is feasible that our method could be used for dnazyme or aptazyme based new sensing method developments for the quantification of other target analytes with high sensitivity and selectivity. (c) 2013 elsevier b.v. all rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000329881100039
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/51055]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li, Wenying,Yang, Yue,Chen, Jian,et al. Detection of lead(II) ions with a DNAzyme and isothermal strand displacement signal amplification[J]. biosensors & bioelectronics,2014,53(1):245-249.
APA	Li, Wenying.,Yang, Yue.,Chen, Jian.,Zhang,Qingfeng.,Wang,Yan.,...&Yu,Cong.(2014).Detection of lead(II) ions with a DNAzyme and isothermal strand displacement signal amplification.biosensors & bioelectronics,53(1),245-249.
MLA	Li, Wenying,et al."Detection of lead(II) ions with a DNAzyme and isothermal strand displacement signal amplification".biosensors & bioelectronics 53.1(2014):245-249.