A putative flowering-time-related Dof transcription factor gene, JcDof3, is controlled by the circadian clock in Jatropha curcas | |
Yang, J. | |
刊名 | Plant Science
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2011 | |
卷号 | 181期号:6页码:667-674 |
ISSN号 | 0168-9452 |
中文摘要 | Plant-specific DNA-binding transcription factors with one finger (Dot) perform important roles in several biological processes. A yeast one-hybrid cDNA library of Jatropha curcas was used to identify Dof-type transcription factors. JcDof3, isolated from the library as a full-length cDNA, encoded a protein of 518 amino acids and contained a highly conserved Dof domain. Yeast one-hybrid systems and subcellular localization assays confirmed that JcDof3 was a typical transcription factor. In contrast to arrhythmic expression at basal level in etiolated cotyledons under continuous dark conditions, the circadian oscillations of JcDoj3 transcripts were observed under long day, short day or continuous light regimes. A phylogenetic analysis showed that JcDof3 was clustered into the same clade with CYCLING DOF FACTOR (CDF), which interacts with F-box protein to regulate photoperiodic flowering. Moreover, a yeast two-hybrid assay showed that JcDof3 also interacted with F-box proteins. Our results suggest that JcDof3 is a circadian clock regulated gene, and might be involved in the flowering time regulation off. curcas. (C) 2011 Elsevier Ireland Ltd. All rights reserved. |
公开日期 | 2015-01-28 |
内容类型 | 期刊论文 |
源URL | [http://ir.xtbg.org.cn/handle/353005/8032] ![]() |
专题 | 西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
推荐引用方式 GB/T 7714 | Yang, J.. A putative flowering-time-related Dof transcription factor gene, JcDof3, is controlled by the circadian clock in Jatropha curcas[J]. Plant Science,2011,181(6):667-674. |
APA | Yang, J..(2011).A putative flowering-time-related Dof transcription factor gene, JcDof3, is controlled by the circadian clock in Jatropha curcas.Plant Science,181(6),667-674. |
MLA | Yang, J.."A putative flowering-time-related Dof transcription factor gene, JcDof3, is controlled by the circadian clock in Jatropha curcas".Plant Science 181.6(2011):667-674. |
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