Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas

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	Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas
	Wei, Q.
刊名	Journal of Plant Physiology
	2012
卷号	169 期号:8 页码:816-824
ISSN号	0176-1617
中文摘要	A cDNA clone encoding a putative beta-ketoacyl-acyl carrier protein (ACP) synthase II (KASII), a key enzyme in fatty acid biosynthesis, was isolated from Jatropha curcas L., a woody oil plant. The isolated cDNA clone of JcKASII contained a 1722-bp open reading frame coding for 573 amino acids with a predicted molecular mass of about 60.98 kDa and the conserved Cys(324) residues that has been proposed as the active site of KASII proteins. The deduced amino acid sequence of the cDNA clone had about 70-84% identity with the KASII from other plants. The transcript of JcKASII was detected in all tissues examined and increased during seed maturation. Expression of JcKASII in the Arabidopsis KASII mutant (fab1) could complement the fatty acid composition of the mutant. Overexpression of JcKASII cDNA under the cauliflower mosaic virus 35S promoter in Arabidopsis resulted in decreasing 16-carbon fatty acids and increasing 18-carbon fatty acids in leaves and seeds. Taken together, these results show that JcKASII could function in 18-carbon fatty acids accumulation in plant and may be useful in the genetic engineering of J. curcas. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7982]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Wei, Q.. Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas[J]. Journal of Plant Physiology,2012,169(8):816-824.
APA	Wei, Q..(2012).Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas.Journal of Plant Physiology,169(8),816-824.
MLA	Wei, Q.."Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas".Journal of Plant Physiology 169.8(2012):816-824.