Upgraded genomic information of Jatropha curcas L
Hirakawa, H.
刊名Plant Biotechnology
2012
卷号29期号:2页码:123-130
ISSN号1342-4580
中文摘要In order to upgrade the genome sequence information of J. curcas L., we integrated de novo assembly of a total of 537 million paired-end reads generated from the Illumina sequencing platform into the current genome assembly which was obtained by a combination of the conventional Sanger method and the Roche/454 sequencing platform. The total length of the upgraded genome sequences thus obtained was 297,661,187 bp consisting of 39,277 contigs. The average and N50 lengths of the generated contigs were 7,579 bp and 15,950 bp, both of which were increased fourfold from the previous genome assembly. Along with genome sequence upgrading, the currently available transcriptome data were collected from the public databases and assembled into 19,454 tentative consensus sequences. Based on a comparison between these tentative consensus sequences of transcripts and the predictions of computer programs, a total of 30,203 complete and partial structures of protein-encoding genes were deduced. The number of genes with complete structures was increased about threefold from the previous genome annotation. By applying the upgraded genome sequence and predicted protein-coding gene information, the number and features of the tandemly arrayed genes, syntenic relations between Jatropha and other plant genomes, and structural features of transposable elements were investigated. The detailed information on the updated J. curcas genome is available at http://www.kazusa.or.jp/jatropha/.
公开日期2015-01-28
内容类型期刊论文
源URL[http://ir.xtbg.org.cn/handle/353005/7874]  
专题西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
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Hirakawa, H.. Upgraded genomic information of Jatropha curcas L[J]. Plant Biotechnology,2012,29(2):123-130.
APA Hirakawa, H..(2012).Upgraded genomic information of Jatropha curcas L.Plant Biotechnology,29(2),123-130.
MLA Hirakawa, H.."Upgraded genomic information of Jatropha curcas L".Plant Biotechnology 29.2(2012):123-130.
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