Characterization of the casbene synthase homolog from Jatropha (Jatropha curcas L.)

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	Characterization of the casbene synthase homolog from Jatropha (Jatropha curcas L.)
	Nakano, Y.
刊名	Plant Biotechnology
	2012
卷号	29 期号:2 页码:185-189
ISSN号	1342-4580
中文摘要	The high oil content of Jatropha (Jatropha curcas L.) seeds makes Jatropha an attractive resource for the production of sustainable bioenergy. However, the Jatropha seed kernels also contain antinutrients and various toxins that persist in the oil and seed cakes and pose a safety risk. Since phorbol esters (PEs) are the major contributor to toxicity, a better understanding of PE biosynthesis is expected to elucidate an effective strategy for the utilization of Jatropha plants. In this study, a Jatropha curcas casbene synthase homolog (JcCSH) with high sequence similarity to casbene synthases (CSs) from Ricinus communis, Euphorbia esula, and Sapium sebiferum was cloned from Jatropha leaf tissue. CS has been hypothesized to catalyze the first step of phorbol biosynthesis. JcCSH encodes a protein that contains a chloroplast transit peptide and a DDXXD motif that is conserved among known terpene cyclases. JcCSH was expressed in seedlings, mature leaves, and the flesh of developing fruits, but not in developing seeds. Our results suggest that JcCSH is widely involved in casbene biosynthesis in various tissues other than seeds.
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7859]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Nakano, Y.. Characterization of the casbene synthase homolog from Jatropha (Jatropha curcas L.)[J]. Plant Biotechnology,2012,29(2):185-189.
APA	Nakano, Y..(2012).Characterization of the casbene synthase homolog from Jatropha (Jatropha curcas L.).Plant Biotechnology,29(2),185-189.
MLA	Nakano, Y.."Characterization of the casbene synthase homolog from Jatropha (Jatropha curcas L.)".Plant Biotechnology 29.2(2012):185-189.