Plant regeneration of non-toxic Jatropha curcas-impacts of plant growth regulators, source and type of explants

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	Plant regeneration of non-toxic Jatropha curcas-impacts of plant growth regulators, source and type of explants
	Kumar, N.
刊名	Journal of Plant Biochemistry and Biotechnology
	2011
卷号	20 期号:1 页码:125-133
ISSN号	0971-7811
中文摘要	Jatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel plant, however, oil and deoiled cake are toxic. A non-toxic variety of J. curcas is reported from Mexico. The present investigation explores the effects of different plant growth regulators (PGRs) viz. 6-benzyl aminopurine (BAP) or thidiazuron (TDZ) individually and in combination with indole-3-butyric acid (IBA), on regeneration from in vitro and field-grown mature leaf explants, in vitro and glasshouse-grown seedlings cotyledonary leaf explants of non-toxic J. curcas. In all the tested parameters maximum regeneration efficiency (81.07%) and the number of shoot buds per explants (20.17) was observed on 9.08 mu M TDZ containing Murashige and Skoog's (MS) medium from in vitro cotyledonary leaf explants. The regenerated shoot buds were transferred to MS medium containing 10 mu M kinetin (Kri), 4.5 mu M BAP and 5.5 mu M alpha-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with 2.25 mu M BAP and 8.5 mu M IAA. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA and NAA for four days followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg/l activated charcoal. The rooted plants could be established in soil with more than 90% survival rate.
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7114]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Kumar, N.. Plant regeneration of non-toxic Jatropha curcas-impacts of plant growth regulators, source and type of explants[J]. Journal of Plant Biochemistry and Biotechnology,2011,20(1):125-133.
APA	Kumar, N..(2011).Plant regeneration of non-toxic Jatropha curcas-impacts of plant growth regulators, source and type of explants.Journal of Plant Biochemistry and Biotechnology,20(1),125-133.
MLA	Kumar, N.."Plant regeneration of non-toxic Jatropha curcas-impacts of plant growth regulators, source and type of explants".Journal of Plant Biochemistry and Biotechnology 20.1(2011):125-133.