Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp JM3 and the evaluation of their antimicrobial properties

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	Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp JM3 and the evaluation of their antimicrobial properties
	Allen, A. D.
刊名	World Journal of Microbiology & Biotechnology
	2012
卷号	28 期号:9 页码:2791-2800
ISSN号	0959-3993
中文摘要	(R)-3-Hydroxycarboxylic acids, chiral enantiomers of bacterial polyhydroxyalkanoates (PHA), may be valuable synthons for the production of numerous industrial materials such as beta-lactams, fungicides, flavors, pheromones and vitamins. In this study, (R)-3-hydroxycarboxylic acid [(R)-3HAs)] synthons were produced by Streptomyces sp. JM3 (JN166713) under batch fermentation. Initial confirmation of PHA production was achieved by matrix assisted laser desorption ionization-time of flight mass spectroscopy and gas chromatography/mass spectroscopy (GC/MS). Subsequently, (R)-3HAs were produced by in vivo depolymerization and the monomers were separated using acid precipitation and anion exchange chromatography. The (R)-3HAs were identified by GC/MS as 3-trimethylsiloxy esters of decanoic, octanoic and butanoic acids. This was further supported by C-13 nuclear magnetic resonance spectrometry. The (R)-3HAs exhibited antimicrobial activity against Escherichia coli O157:H7, Listeria monocytogenes (ATCC 7644) and Salmonella typhimurium (ATCC 14028) with minimum inhibitory concentration ranging from 12.5 to 25 mg ml(-1). However, the minimum bactericidal concentration data suggest that the (R)-3HAs may be bactericidal for E. coli O157:H7 and bacteriostatic for S. typhimurium and L. monocytogenes. Furthermore, the major purified synthon was shown to minimize the invasion of fibroblasts by S. typhimurium (ATCC 14028) [p < 0.05], using the MTT assay [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)].
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7109]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Allen, A. D.. Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp JM3 and the evaluation of their antimicrobial properties[J]. World Journal of Microbiology & Biotechnology,2012,28(9):2791-2800.
APA	Allen, A. D..(2012).Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp JM3 and the evaluation of their antimicrobial properties.World Journal of Microbiology & Biotechnology,28(9),2791-2800.
MLA	Allen, A. D.."Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp JM3 and the evaluation of their antimicrobial properties".World Journal of Microbiology & Biotechnology 28.9(2012):2791-2800.