Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 热带植物资源可持续利用重点实验室特色文献 > 小桐子相关文献

	Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant
	Zhang, C.
刊名	In Vitro Cellular & Developmental Biology-Plant
	2013
卷号	49 期号:5 页码:529-540
ISSN号	1054-5476
中文摘要	In order to establish a highly efficient and sustainable regeneration system, we systematically researched the key factors affecting direct shoot regeneration from Jatropha curcas leaves that were collected from Hainan (HN1-1), Lijiang (LJ3-1), and Yuxi (YX2-12) provinces in China. The L-9(3(4)) orthogonal test of thidiazuron (TDZ), kinetin (Kn), and gibberellic acid (GA(3)) were studied, and the explant type, growth age, and cultivar of leaves were subsequently investigated. Simultaneously, the combinations of plant growth regulators (PGRs) promoting shoot bud proliferation, elongation, and root establishment were examined. The results showed that the best medium for shoot bud induction was Murashige and Skoog (MS) medium supplemented with 1.0 mg/L TDZ, 0.5 mg/L Kn, and 0.5 mg/L GA(3). TDZ was the key PGR, while Kn and GA(3) played an important role in shoot bud elongation and the number of shoots per leaf disk, respectively. The induced shoot buds proliferated and readily elongated in MS medium with 0.3 mg/L 6-benzylaminopurine and 0.01 mg/L indole-3-butyric acid (IBA) and established roots in half-strength MS medium supplemented with 2.0 mg/L IBA. Using the previously described methods, the third to fifth leaves were found to be the best explant source for shoot bud induction, with a high induction rate, large shoot numbers per disk, excellent proliferation, and consistent rooting. With the use of this regeneration system, the shoot bud induction rate increased from the reported rate of 53.5% to more than 90% using different explants and cultivars, and the shoot number per leaf disk (shoot length a parts per thousand yenaEuro parts per thousand 0.5 cm) increased from 1.6 to 3.5. Thus, this optimized regeneration system will effectively promote the propagation and genetic transformation of J. curcas.
语种	英语
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7059]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Zhang, C.. Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant[J]. In Vitro Cellular & Developmental Biology-Plant,2013,49(5):529-540.
APA	Zhang, C..(2013).Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant.In Vitro Cellular & Developmental Biology-Plant,49(5),529-540.
MLA	Zhang, C.."Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant".In Vitro Cellular & Developmental Biology-Plant 49.5(2013):529-540.