A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress

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	A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress
	Zhang, F. L.
刊名	Plant Science
	2008
卷号	174 期号:5 页码:510-518
ISSN号	0168-9452
中文摘要	A novel betaine aldehyde dehydrogenase gene, named JcBD1, was cloned by RT-PCR and RACE techniques from Jatropha curcas L., an important plant species as its seed is the raw material for biodiesels. The cDNA was 1769 bp in length with a complete open reading frame of 1509 bp, encoding 503 amino acids with a predicted molecular mass of 54.585 kD and a pI of 5.32. The deduced amino acid sequence showed substantial similarities to BADH enzymes present in other plants, including the absolute conservative region in the possible active site. Southern blot analysis indicated that the JcBD1 gene belongs to a multigene family. Semi-quantitative RT-PCR analyses revealed that JcBD1 gene was expressed in roots, stems, leaves, flowers, and young seeds, but that the expression level was highest in leaves and stems. And the JcBD1 gene was increasedly expressed in leaves undergoing environmental stress like drought (30% PEG), heat (50 T), and salt (300 MM NaCl). The JcBD1 protein was functionally expressed in Escherichia coli (E. coli). E. coli cells transformed with the JcBD1 gene obviously grew better than control cells. The enzyme activity of expressed JcBD1 protein was detected. Over-expression of JcBD1 enzyme in E. coli conferred its resistance to abiotic stressors like salt. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
语种	英语
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7031]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Zhang, F. L.. A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress[J]. Plant Science,2008,174(5):510-518.
APA	Zhang, F. L..(2008).A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress.Plant Science,174(5),510-518.
MLA	Zhang, F. L.."A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress".Plant Science 174.5(2008):510-518.