Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes

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	Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes
	Li, Guangyue; Ren, Jie; Wu, Qiaqing; Feng, Jinhui; Zhu, Dunming; Ma, Yanhe
刊名	JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
	2013-06-01
卷号	90 期号:90 页码:17-22
关键词	Oceanospirillum sp. Aldehyde reductase Bioreduction Chemoselectivity Marine enzyme
英文摘要	A putative aldehyde reductase gene from Oceanospirillum sp. MED92 was overexpressed in Escherichia coli. The recombinant protein (OsAR) was characterized as a monomeric NADPH-dependent aldehyde reductase. The kinetic parameters K-m and k(cat) of OsAR were 0.89 +/- 0.08 mM and 11.07 +/- 0.99 s(-1) for benzaldehyde, 0.04 +/- 0.01 mM and 6.05 +/- 1.56 s(-1) for NADPH, respectively. This enzyme exhibited high activity toward a variety of aromatic and aliphatic aldehydes, but no activity toward ketones. As such, it catalyzed the chemoselective reduction of aldehydes in the presence of ketones, as demonstrated by the reduction of 4-acetylbenzaldehyde or the mixture of hexanal and 2-nonanone, showing the application potential of this marine enzyme in such selective reduction of synthetic importance. (C) 2013 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Physical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	ALDO-KETO REDUCTASES ; SPOROBOLOMYCES-SALMONICOLOR ; SUBSTRATE-SPECIFICITY ; AROMATIC-ALDEHYDES ; EUTYPA-LATA ; PURIFICATION ; SUPERFAMILY ; BIOCATALYSTS ; TOXIN ; DEHYDROGENASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000316924800003
公开日期	2015-01-24
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/311007/714]
专题	天津工业生物技术研究所_生物催化与绿色化工朱敦明_期刊论文
作者单位	Chinese Acad Sci, Tianjin Airport Econ Area, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Li, Guangyue,Ren, Jie,Wu, Qiaqing,et al. Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2013,90(90):17-22.
APA	Li, Guangyue,Ren, Jie,Wu, Qiaqing,Feng, Jinhui,Zhu, Dunming,&Ma, Yanhe.(2013).Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,90(90),17-22.
MLA	Li, Guangyue,et al."Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 90.90(2013):17-22.